ArticleNature methods2014
Accurate macromolecular structures using minimal measurements from X-ray free-electron lasers.
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Article in Nature methods, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 105 papers.
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105citing papers in PubMed
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Who cites it
105 citing papers in PubMed.
- Polyethylene Terephthalate Film-Based Sample Holder for Fixed-Target Serial Femtosecond Crystallography.Materials (Basel, Switzerland) · 2026Article
- Drop-on-fixed-target reaction initiation approach for serial and time-resolved crystallography.IUCrJ · 2026Article
- Advances in Crystallography and Cryo-Electron Microscopy for the Study of Membrane Proteins.Advances in experimental medicine and biology · 2026Review
- Standard Sample Preparation for Serial Femtosecond Crystallography.Biomolecules · 2025Review
- Serial-femtosecond crystallography reveals how a phytochrome variant couples chromophore and protein structural changes.Science advances · 2025Article
- Article
- Macromolecular crystallography and biology at the Linac Coherent Light Source.Journal of synchrotron radiation · 2025Article
- Robust error calibration for serial crystallography.Acta crystallographica. Section D, Structural biology · 2025Article
- GARFIELD, a toolkit for interpreting ultrafast electron diffraction data of imperfect quasi-single crystals.Structural dynamics (Melville, N.Y.) · 2025Article
- Filling data analysis gaps in time-resolved crystallography by machine learning.Structural dynamics (Melville, N.Y.) · 2025Article
- A snapshot love story: what serial crystallography has done and will do for us.Acta crystallographica. Section D, Structural biology · 2024Review
- The evolution of raw data archiving and the growth of its importance in crystallography.IUCrJ · 2024Article
- Changes in an enzyme ensemble during catalysis observed by high-resolution XFEL crystallography.Science advances · 2024Article
- In Situ Structural Observation of a Substrate- and Peroxide-Bound High-Spin Ferric-Hydroperoxo Intermediate in the P450 Enzyme CYP121.Journal of the American Chemical Society · 2023Article
- Going around the Kok cycle of the water oxidation reaction with femtosecond X-ray crystallography.IUCrJ · 2023Article
- Structure of a ribonucleotide reductase R2 protein radical.Science (New York, N.Y.) · 2023Article
- Changes in an Enzyme Ensemble During Catalysis Observed by High Resolution XFEL Crystallography.bioRxiv : the preprint server for biology · 2023Article
- Growing and making nano- and microcrystals.Nature protocols · 2023Review
- Capturing the sequence of events during the water oxidation reaction in photosynthesis using XFELs.FEBS letters · 2023Review
- Room temperature crystallography and X-ray spectroscopy of metalloenzymes.Methods in enzymology · 2023Article
45 more citing papers are in PubMed but not listed here.
Corrections and comments
- Erratum issued
Authors and funding
41 authors.
Johan HattnePhysical Biosciences Division, Lawrence Berkeley National Laboratory, Berkeley, California, USA.
Nathaniel EcholsPhysical Biosciences Division, Lawrence Berkeley National Laboratory, Berkeley, California, USA.
Rosalie TranPhysical Biosciences Division, Lawrence Berkeley National Laboratory, Berkeley, California, USA.
Jan KernPhysical Biosciences Division, Lawrence Berkeley National Laboratory, Berkeley, California, USA.
Richard J Gildea1] Physical Biosciences Division, Lawrence Berkeley National Laboratory, Berkeley, California, USA. [2].
Aaron S BrewsterPhysical Biosciences Division, Lawrence Berkeley National Laboratory, Berkeley, California, USA.
Roberto Alonso-MoriLinac Coherent Light Source (LCLS), SLAC National Accelerator Laboratory, Menlo Park, California, USA.ORCID 0000000253570934
Carina GlöcknerMax-Volmer-Laboratorium für Biophysikalische Chemie, Technische Universität, Berlin, Germany.
Julia HellmichMax-Volmer-Laboratorium für Biophysikalische Chemie, Technische Universität, Berlin, Germany.
Hartawan LaksmonoStanford PULSE Institute, SLAC National Accelerator Laboratory, Menlo Park, California, USA.
Raymond G SierraStanford PULSE Institute, SLAC National Accelerator Laboratory, Menlo Park, California, USA.
Benedikt Lassalle-KaiserPhysical Biosciences Division, Lawrence Berkeley National Laboratory, Berkeley, California, USA.
Alyssa LampePhysical Biosciences Division, Lawrence Berkeley National Laboratory, Berkeley, California, USA.
Guangye HanPhysical Biosciences Division, Lawrence Berkeley National Laboratory, Berkeley, California, USA.
Sheraz GulPhysical Biosciences Division, Lawrence Berkeley National Laboratory, Berkeley, California, USA.
Dörte DiFioreMax-Volmer-Laboratorium für Biophysikalische Chemie, Technische Universität, Berlin, Germany.
Despina MilathianakiLinac Coherent Light Source (LCLS), SLAC National Accelerator Laboratory, Menlo Park, California, USA.
Alan R FryLinac Coherent Light Source (LCLS), SLAC National Accelerator Laboratory, Menlo Park, California, USA.
Alan MiahnahriLinac Coherent Light Source (LCLS), SLAC National Accelerator Laboratory, Menlo Park, California, USA.
William E WhiteLinac Coherent Light Source (LCLS), SLAC National Accelerator Laboratory, Menlo Park, California, USA.
Donald W SchaferLinac Coherent Light Source (LCLS), SLAC National Accelerator Laboratory, Menlo Park, California, USA.
M Marvin SeibertLinac Coherent Light Source (LCLS), SLAC National Accelerator Laboratory, Menlo Park, California, USA.
Jason E KoglinLinac Coherent Light Source (LCLS), SLAC National Accelerator Laboratory, Menlo Park, California, USA.
Dimosthenis SokarasStanford Synchrotron Radiation Lightsource, SLAC National Accelerator Laboratory, Menlo Park, California, USA.
Tsu-Chien WengStanford Synchrotron Radiation Lightsource, SLAC National Accelerator Laboratory, Menlo Park, California, USA.
Jonas Sellberg1] Stanford Synchrotron Radiation Lightsource, SLAC National Accelerator Laboratory, Menlo Park, California, USA. [2] Department of Physics, AlbaNova, Stockholm University, Stockholm, Sweden.
Matthew J LatimerStanford Synchrotron Radiation Lightsource, SLAC National Accelerator Laboratory, Menlo Park, California, USA.
Pieter GlatzelEuropean Synchrotron Radiation Facility, Grenoble, France.
Petrus H ZwartPhysical Biosciences Division, Lawrence Berkeley National Laboratory, Berkeley, California, USA.
Ralf W Grosse-KunstlevePhysical Biosciences Division, Lawrence Berkeley National Laboratory, Berkeley, California, USA.
Michael J Bogan1] Linac Coherent Light Source (LCLS), SLAC National Accelerator Laboratory, Menlo Park, California, USA. [2] Stanford PULSE Institute, SLAC National Accelerator Laboratory, Menlo Park, California, USA.
Marc MesserschmidtLinac Coherent Light Source (LCLS), SLAC National Accelerator Laboratory, Menlo Park, California, USA.
Garth J WilliamsLinac Coherent Light Source (LCLS), SLAC National Accelerator Laboratory, Menlo Park, California, USA.
Sébastien BoutetLinac Coherent Light Source (LCLS), SLAC National Accelerator Laboratory, Menlo Park, California, USA.
Johannes MessingerInstitutionen för Kemi, Kemiskt Biologiskt Centrum, Umeå Universitet, Umeå, Sweden.
Athina Zouni1] Max-Volmer-Laboratorium für Biophysikalische Chemie, Technische Universität, Berlin, Germany. [2] Institut für Biologie, Humboldt Universität zu Berlin, Berlin, Germany.
Junko YanoPhysical Biosciences Division, Lawrence Berkeley National Laboratory, Berkeley, California, USA.
Uwe BergmannLinac Coherent Light Source (LCLS), SLAC National Accelerator Laboratory, Menlo Park, California, USA.
Vittal K YachandraPhysical Biosciences Division, Lawrence Berkeley National Laboratory, Berkeley, California, USA.
Paul D AdamsPhysical Biosciences Division, Lawrence Berkeley National Laboratory, Berkeley, California, USA.
Nicholas K SauterPhysical Biosciences Division, Lawrence Berkeley National Laboratory, Berkeley, California, USA.
Funding
X-ray Absorption Spectroscopy (XAS) pp.711-759P41GM103393 · NIGMS · STANFORD UNIVERSITY · PI HODGSON, KEITH O · 2012 to 2019
$30.6MTaking Snapshots of the Water-Oxidation Reaction by the Mn4Ca Oxygen-Evolving Complex Using X-ray Crystallography and SpectroscopyR01GM055302 · NIGMS · UNIVERSITY OF CALIF-LAWRENC BERKELEY LAB · PI YACHANDRA, VITTAL · 1997 to 2022
$9.1MBiological Spectroscopy and Crystallography Using an X-ray Free Electron Laser - Supplemental EquipmentR01GM110501 · NIGMS · UNIVERSITY OF CALIF-LAWRENC BERKELEY LAB · PI Junko Yano · 2014 to 2026
$4.8MRealizing New Horizons in X-ray Crystallography Data ProcessingR01GM095887 · NIGMS · UNIVERSITY OF CALIF-LAWRENC BERKELEY LAB · PI SAUTER, NICHOLAS K · 2011 to 2014
$1.5MTowards real-time XFEL data reduction with CCTBXR01GM102520 · NIGMS · UNIVERSITY OF CALIF-LAWRENC BERKELEY LAB · PI SAUTER, NICHOLAS K · 2012 to 2015
$1.4MStructure and Function of the Mn Oxygen-Evolving ComplexR56GM055302 · NIGMS · UNIVERSITY OF CALIF-LAWRENC BERKELEY LAB · PI YACHANDRA, VITTAL · 2009 to 2009
$530kNIGMS NIH HHS GM055302NIGMS NIH HHS GM095887NIGMS NIH HHS GM102520NIGMS NIH HHS P41 GM103393NIGMS NIH HHS P41GM103393NIGMS NIH HHS R01 GM055302NIGMS NIH HHS R01 GM095887NIGMS NIH HHS R01 GM102520NIGMS NIH HHS R01 GM110501NIGMS NIH HHS R56 GM055302
Abstract
X-ray free-electron laser (XFEL) sources enable the use of crystallography to solve three-dimensional macromolecular structures under native conditions and without radiation damage. Results to date, however, have been limited by the challenge of deriving accurate Bragg intensities from a heterogeneous population of microcrystals, while at the same time modeling the X-ray spectrum and detector geometry. Here we present a computational approach designed to extract meaningful high-resolution signals from fewer diffraction measurements.
Indexed as
LasersBacillusCalciumCalibrationComputer SimulationCrystallizationCrystallography, X-RayElectronsEquipment DesignLikelihood FunctionsMacromolecular SubstancesModels, ChemicalMolecular ConformationMuramidaseNanotechnologyReproducibility of ResultsCalciumMacromolecular SubstancesMuramidaseThermolysinZinc
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