ArticlePloS one2014
High content image analysis identifies novel regulators of synaptogenesis in a high-throughput RNAi screen of primary neurons.
Article in PloS one, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
27 citing papers in PubMed, 48 citations in OpenAlex.
- A human-specific microRNA controls the timing of excitatory synaptogenesis.Nature communications · 2026Article
- Review
- High-content image-based pooled screens reveal regulators of synaptogenesis.Cell reports · 2025Article
- Disease phenotypic screening in neuron-glia cocultures identifies blockers of inflammatory neurodegeneration.iScience · 2024Article
- Article
- Identification of the common neurobiological process disturbed in genetic and non-genetic models for autism spectrum disorders.Cellular and molecular life sciences : CMLS · 2022Article
- High-throughput Analysis of Synaptic Activity in Electrically Stimulated Neuronal Cultures.Neuroinformatics · 2021Article
- Article
- Bryostatin 1 Promotes Synaptogenesis and Reduces Dendritic Spine Density in Cortical Cultures through a PKC-Dependent Mechanism.ACS chemical neuroscience · 2020Article
- Live Neuron High-Content Screening Reveals Synaptotoxic Activity in Alzheimer Mouse Model Homogenates.Scientific reports · 2020Article
- CRISPR Interference-Based Platform for Multimodal Genetic Screens in Human iPSC-Derived Neurons.Neuron · 2019Article
- Multiplexed and high-throughput neuronal fluorescence imaging with diffusible probes.Nature communications · 2019Article
- High-throughput microscopy exposes a pharmacological window in which dual leucine zipper kinase inhibition preserves neuronal network connectivity.Acta neuropathologica communications · 2019Article
- DoGNet: A deep architecture for synapse detection in multiplexed fluorescence images.PLoS computational biology · 2019Article
- Automated Live-Cell Imaging of Synapses in Rat and Human Neuronal Cultures.Frontiers in cellular neuroscience · 2019Article
- Improved Scalability of Neuron-Based Phenotypic Screening Assays for Therapeutic Discovery in Neuropsychiatric Disorders.Molecular neuropsychiatry · 2018Article
- Image-Based Profiling of Synaptic Connectivity in Primary Neuronal Cell Culture.Frontiers in neuroscience · 2018Review
- Using High-Throughput Animal or Cell-Based Models to Functionally Characterize GWAS Signals.Current genetic medicine reports · 2018Review
- Myostatin-like proteins regulate synaptic function and neuronal morphology.Development (Cambridge, England) · 2017Article
- The application of CRISPR technology to high content screening in primary neurons.Molecular and cellular neurosciences · 2017Article
Corrections and comments
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Authors and funding
8 authors at 2 institutions in 1 country.
Funding
Abstract
The formation of synapses, the specialized points of chemical communication between neurons, is a highly regulated developmental process fundamental to establishing normal brain circuitry. Perturbations of synapse formation and function causally contribute to human developmental and degenerative neuropsychiatric disorders, such as Alzheimer's disease, intellectual disability, and autism spectrum disorders. Many genes controlling synaptogenesis have been identified, but lack of facile experimental systems has made systematic discovery of regulators of synaptogenesis challenging. Thus, we created a high-throughput platform to study excitatory and inhibitory synapse development in primary neuronal cultures and used a lentiviral RNA interference library to identify novel regulators of synapse formation. This methodology is broadly applicable for high-throughput screening of genes and drugs that may rescue or improve synaptic dysfunction associated with cognitive function and neurological disorders.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.