ArticlePloS one2014
Acellular bi-layer silk fibroin scaffolds support tissue regeneration in a rabbit model of onlay urethroplasty.
Article in PloS one, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 32 papers, 2 of them syntheses that pooled it.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
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Who cites it
32 citing papers in PubMed, 2 syntheses or guidelines pooled it, 52 citations in OpenAlex.
- Tissue engineering of urethra: Systematic review of recent literature.Experimental biology and medicine (Maywood, N.J.) · 2017Pooled it
- A systematic review of animal and clinical studies on the use of scaffolds for urethral repair.Journal of Huazhong University of Science and Technology. Medical sciences = Hua zhong ke ji da xue xue bao. Yi xue Ying De wen ban = Huazhong keji daxue xuebao. Yixue Yingdewen ban · 2016Pooled it
- Evolution and innovation in urethroplasty: a comprehensive narrative review of graft types and surgical techniques.International journal of impotence research · 2026Review
- Two is Better than One: Evaluation of Single Versus Staged Approaches for Tubular Substitution Urethroplasty with Acellular Silk Fibroin Biomaterials in Rabbits.Tissue engineering and regenerative medicine · 2025Article
- Evaluation of Bi-layer Silk Fibroin Grafts for Onlay Urethroplasty in a Female Porcine Model of Long Urethral Strictures.Tissue engineering and regenerative medicine · 2025Article
- Evaluation of Bi-layer Silk Fibroin Grafts for Inlay Vaginoplasty in a Rat Model.Tissue engineering and regenerative medicine · 2024Article
- Tissue Engineering for Penile Reconstruction.Bioengineering (Basel, Switzerland) · 2024Review
- Silk fibroin-based scaffolds for tissue engineering.Frontiers in bioengineering and biotechnology · 2024Review
- Development of male and female models of long urethral strictures in swine.Surgery open science · 2023Article
- Fibrinogen/poly(l-lactide-co-caprolactone) copolymer scaffold: A potent adhesive material for urethral tissue regeneration in urethral injury treatment.Regenerative therapy · 2023Article
- Evaluation of silk fibroin-based urinary conduits in a porcine model of urinary diversion.Frontiers in bioengineering and biotechnology · 2023Article
- Tailor-made natural and synthetic grafts for precise urethral reconstruction.Journal of nanobiotechnology · 2022Review
- Tissue Engineering and Regenerative Medicine in Pediatric Urology: Urethral and Urinary Bladder Reconstruction.International journal of molecular sciences · 2022Review
- Genitourinary Tissue Engineering: Reconstruction and Research Models.Bioengineering (Basel, Switzerland) · 2021Review
- Mechanical, compositional and morphological characterisation of the human male urethra for the development of a biomimetic tissue engineered urethral scaffold.Biomaterials · 2021Article
- Evaluation of Bi-Layer Silk Fibroin Grafts for Tubular Ureteroplasty in a Porcine Defect Model.Frontiers in bioengineering and biotechnology · 2021Article
- Evaluation of Bi-Layer Silk Fibroin Grafts for Penile Tunica Albuginea Repair in a Rabbit Corporoplasty Model.Frontiers in bioengineering and biotechnology · 2021Article
- The current state of tissue engineering in the management of hypospadias.Nature reviews. Urology · 2020Review
- Electrospinning: Application and Prospects for Urologic Tissue Engineering.Frontiers in bioengineering and biotechnology · 2020Review
- Overview of Urethral Reconstruction by Tissue Engineering: Current Strategies, Clinical Status and Future Direction.Tissue engineering and regenerative medicine · 2019Review
Corrections and comments
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Authors and funding
9 authors at 3 institutions in 1 country.
Funding
Abstract
Acellular scaffolds derived from Bombyx mori silk fibroin were investigated for their ability to support functional tissue regeneration in a rabbit model of urethra repair. A bi-layer silk fibroin matrix was fabricated by a solvent-casting/salt leaching process in combination with silk fibroin film casting to generate porous foams buttressed by homogeneous silk fibroin films. Ventral onlay urethroplasty was performed with silk fibroin grafts (Group 1, N = 4) (Width × Length, 1 × 2 cm(2)) in adult male rabbits for 3 m of implantation. Parallel control groups consisted of animals receiving small intestinal submucosa (SIS) implants (Group 2, N = 4) or urethrotomy alone (Group 3, N = 3). Animals in all groups exhibited 100% survival prior to scheduled euthanasia and achieved voluntary voiding following 7 d of initial catheterization. Retrograde urethrography of each implant group at 3 m post-op revealed wide urethral calibers and preservation of organ continuity similar to pre-operative and urethrotomy controls with no evidence of contrast extravasation, strictures, fistulas, or stone formation. Histological (hematoxylin and eosin and Masson's trichrome), immunohistochemical, and histomorphometric analyses demonstrated that both silk fibroin and SIS scaffolds promoted similar extents of smooth muscle and epithelial tissue regeneration throughout the original defect sites with prominent contractile protein (α-smooth muscle actin and SM22α) and cytokeratin expression, respectively. De novo innervation and vascularization were also evident in all regenerated tissues indicated by synaptophysin-positive neuronal cells and vessels lined with CD31 expressing endothelial cells. Following 3 m post-op, minimal acute inflammatory reactions were elicited by silk fibroin scaffolds characterized by the presence of eosinophil granulocytes while SIS matrices promoted chronic inflammatory responses indicated by mobilization of mononuclear cell infiltrates. The results of this study demonstrate that bi-layer silk fibroin scaffolds represent promising biomaterials for onlay urethroplasty, capable of promoting similar degrees of tissue regeneration in comparison to conventional SIS scaffolds, but with reduced immunogenicity.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.