ArticlePloS one2014
A population of Langerin-positive dendritic cells in murine Peyer's patches involved in sampling β-glucan microparticles.
Article in PloS one, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
17 citing papers in PubMed, 59 citations in OpenAlex.
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- The effects of different dietary fiber pectin structures on the gastrointestinal immune barrier: impact via gut microbiota and direct effects on immune cells.Experimental & molecular medicine · 2020Review
- Candida albicans Elicits Pro-Inflammatory Differential Gene Expression in Intestinal Peyer's Patches.Mycopathologia · 2019Article
- RNA isolation from Peyer's patch lymphocytes and mononuclear phagocytes to determine gene expression profiles using NanoString technology.Journal of biological methods · 2018Article
- Effects of food-borne nanomaterials on gastrointestinal tissues and microbiota.Wiley interdisciplinary reviews. Nanomedicine and nanobiotechnology · 2018Review
- Biomimetic thiamine- and niacin-decorated liposomes for enhanced oral delivery of insulin.Acta pharmaceutica Sinica. B · 2018Article
- A β-Glucan-Based Dietary Fiber Reduces Mast Cell-Induced Hyperpermeability in Ileum From Patients With Crohn's Disease and Control Subjects.Inflammatory bowel diseases · 2017Article
- Characterization of the Probiotic Yeast Saccharomyces boulardii in the Healthy Mucosal Immune System.PloS one · 2016Article
- The Agaricus blazei-Based Mushroom Extract, Andosan™, Protects against Intestinal Tumorigenesis in the A/J Min/+ Mouse.PloS one · 2016Article
- Duality of β-glucan microparticles: antigen carrier and immunostimulants.International journal of nanomedicine · 2016Article
- Molecular Interactions of β-(1→3)-Glucans with Their Receptors.Molecules (Basel, Switzerland) · 2015Review
- Phenotypic Analysis of a Population of IgA+ Cells in the Follicle-Associated Epithelium of Mouse Peyer's Patches.PloS one · 2015Article
- Polymeric nanoparticle drug delivery technologies for oral delivery applications.Expert opinion on drug delivery · 2015Review
- Polymeric nanoparticle technologies for oral drug delivery.Clinical gastroenterology and hepatology : the official clinical practice journal of the American Gastroenterological Association · 2014Review
Corrections and comments
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Authors and funding
5 authors at 4 institutions in 1 country.
Funding
Abstract
Glucan particles (GPs) are 2-4 μm hollow, porous shells composed of 1,3-β-D-glucan that have been effectively used for oral targeted-delivery of a wide range of payloads, including small molecules, siRNA, DNA, and protein antigens. While it has been demonstrated that the transepithelial transport of GPs is mediated by Peyer's patch M cells, the fate of the GPs once within gut-associated lymphoid tissue (GALT) is not known. Here we report that fluorescently labeled GPs administered to mice by gavage accumulate in CD11c+ DCs situated in Peyer's patch sub-epithelial dome (SED) regions. GPs appeared in DCs within minutes after gavage and remained within the SED for days afterwards. The co-administration or sequential administration of GPs with differentially labeled GPs or poly(lactic-co-glycolic acid) nanoparticles demonstrated that the SED DC subpopulation in question was capable of internalizing particles of different sizes and material compositions. Phenotypic analysis identified the GP-containing DCs as being CD8α- and CD11blo/-, suggesting they are the so-called myeloid and/or double negative (DN) subset(s) of PP DCs. A survey of C-type lectin receptors (CLRs) known to be expressed by leukocytes within the intestinal mucosa revealed that GP-containing SED DCs were positive for Langerin (CD207), a CLR with specificity for β-D-glucan and that has been shown to mediate the internalization of a wide range of microbial pathogens, including bacteria, viruses and fungi. The presence of Langerin+ DCs in the SED as determined by immunofluorescence was confirmed using Langerin E-GFP transgenic mice. In summary, our results demonstrate that following M cell-mediated transepithelial transport, GPs (and other micro/nanoparticles) are sampled by a population of SED DCs distinguished from other Peyer's patch DC subsets by their expression of Langerin. Future studies will be aimed at defining the role of Langerin in antigen sampling and antigen presentation within the context of the GALT.
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