ArticlePloS one2014
Periodic heat shock accelerated the chondrogenic differentiation of human mesenchymal stem cells in pellet culture.
Article in PloS one, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed.
- Heat shock proteins in osteoarthritis: molecular mechanisms, pathogenic roles, and therapeutic opportunities.Frontiers in immunology · 2025Review
- Osteoclasts and osteoarthritis: Novel intervention targets and therapeutic potentials during aging.Aging cell · 2024Review
- Low-oxygen tension augments chondrocyte sensitivity to biomimetic thermomechanical cues in cartilage-engineered constructs.iScience · 2023Article
- Prussian Blue Nanoparticle-Entrapped GelMA Gels Laden with Mesenchymal Stem Cells as Prospective Biomaterials for Pelvic Floor Tissue Repair.International journal of molecular sciences · 2023Article
- Strategies to improve regenerative potential of mesenchymal stem cells.World journal of stem cells · 2021Review
- Travelling ultrasound promotes vasculogenesis of three-dimensional-monocultured human umbilical vein endothelial cells.Biotechnology and bioengineering · 2021Article
- Heat Shock Proteins and their Protective Roles in Stem Cell Biology.Stem cell reviews and reports · 2019Review
- Physical Stimulations for Bone and Cartilage Regeneration.Regenerative engineering and translational medicine · 2018Article
- Downregulation of Heat Shock Protein 70 Impairs Osteogenic and Chondrogenic Differentiation in Human Mesenchymal Stem Cells.Scientific reports · 2018Article
- Red (660 nm) or near-infrared (810 nm) photobiomodulation stimulates, while blue (415 nm), green (540 nm) light inhibits proliferation in human adipose-derived stem cells.Scientific reports · 2017Article
- Species-specific developmental timing is maintained by pluripotent stem cells ex utero.Developmental biology · 2017Article
- In vitro chondrogenesis of Wharton's jelly mesenchymal stem cells in hyaluronic acid-based hydrogels.Cellular & molecular biology letters · 2016Article
- Polymodal Transient Receptor Potential Vanilloid (TRPV) Ion Channels in Chondrogenic Cells.International journal of molecular sciences · 2015Article
- Culture temperature affects human chondrocyte messenger RNA expression in monolayer and pellet culture systems.PloS one · 2015Article
Corrections and comments
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Authors and funding
3 authors.
Funding
Abstract
Osteoarthritis (OA) is one of diseases that seriously affect elderly people's quality of life. Human mesenchymal stem cells (hMSCs) offer a potential promise for the joint repair in OA patients. However, chondrogenic differentiation from hMSCs in vitro takes a long time (∼ 6 weeks) and differentiated cells are still not as functionally mature as primary isolated chondrocytes, though chemical stimulations and mechanical loading have been intensively studied to enhance the hMSC differentiation. On the other hand, thermal stimulations of hMSC chondrogenesis have not been well explored. In this study, the direct effects of mild heat shock (HS) on the differentiation of hMSCs into chondrocytes in 3D pellet culture were investigated. Periodic HS at 41 °C for 1 hr significantly increased sulfated glycosaminoglycan in 3D pellet culture at Day 10 of chondrogenesis. Immunohistochemical and Western Blot analyses revealed an increased expression of collagen type II and aggrecan in heat-shocked pellets than non heat-shocked pellets on Day 17 of chondrogenesis. In addition, HS also upregulated the expression of collagen type I and X as well as heat shock protein 70 on Day 17 and 24 of differentiation. These results demonstrate that HS accelerated the chondrogenic differentiation of hMSCs and induced an early maturation of chondrocytes differentiated from hMSCs. The results of this study will guide the design of future protocols using thermal treatments to facilitate cartilage regeneration with human mesenchymal stem cells.
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