ArticleThe Journal of cell biology1989
Isolation of cell-to-cell adherens junctions from rat liver.
Article in The Journal of cell biology, 1989. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 45 papers.
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Who cites it
45 citing papers in PubMed, 119 citations in OpenAlex.
- Phase separation of an actin nucleator by junctional microtubules regulates epithelial function.Science advances · 2023Article
- Buttons and Zippers: Endothelial Junctions in Lymphatic Vessels.Cold Spring Harbor perspectives in medicine · 2022Review
- Synaptopodin stress fiber and contractomere at the epithelial junction.The Journal of cell biology · 2022Article
- A microtubule-LUZP1 association around tight junction promotes epithelial cell apical constriction.The EMBO journal · 2021Article
- Desmosome architecture derived from molecular dynamics simulations and cryo-electron tomography.Proceedings of the National Academy of Sciences of the United States of America · 2020Article
- The desmosome is a mesoscale lipid raft-like membrane domain.Molecular biology of the cell · 2019Article
- Mesenchymal-epithelial transition in development and reprogramming.Nature cell biology · 2019Review
- Synaptopodin couples epithelial contractility to α-actinin-4-dependent junction maturation.The Journal of cell biology · 2015Article
- Conceptual barriers to understanding physical barriers.Seminars in cell & developmental biology · 2015Review
- FSGS3/CD2AP is a barbed-end capping protein that stabilizes actin and strengthens adherens junctions.The Journal of cell biology · 2013Article
- The association of microtubules with tight junctions is promoted by cingulin phosphorylation by AMPK.The Journal of cell biology · 2013Article
- Claudin-5 is involved in breast cancer cell motility through the N-WASP and ROCK signalling pathways.Journal of experimental & clinical cancer research : CR · 2012Article
- Claudin-5 participates in the regulation of endothelial cell motility.Molecular and cellular biochemistry · 2012Article
- Tara up-regulates E-cadherin transcription by binding to the Trio RhoGEF and inhibiting Rac signaling.The Journal of cell biology · 2011Article
- Discovering the molecular components of intercellular junctions--a historical view.Cold Spring Harbor perspectives in biology · 2009Review
- Review
- The keratin-binding protein Albatross regulates polarization of epithelial cells.The Journal of cell biology · 2008Article
- Shoichiro Tsukita: a life exploring the molecular architecture of the tight junction.The Journal of cell biology · 2006Article
- Endocytosis of E-cadherin regulated by Rac and Cdc42 small G proteins through IQGAP1 and actin filaments.The Journal of cell biology · 2004Article
- Role of paracellular junction complexes in baculovirus-mediated gene transfer to nondividing rat hepatocytes.Gene therapy · 2003Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
A new isolation procedure for cell-to-cell adherens junctions has been developed using rat liver. From the bile canaliculi-enriched fraction obtained by homogenization of the liver and sucrose gradient centrifugation, the fraction rich in adherens junction was recovered by detergent treatment followed by sucrose gradient centrifugation. Light and electron microscopy revealed that this final fraction was mainly composed of the belt-like adherens junctions with their associated short actin filaments. Biochemical and immunological analyses have shown that vinculin is highly enriched in this fraction. Considering that vinculin is known to be localized in the cell-to-cell adherens junctions, we can conclude that we have succeeded in isolating the cell-to-cell adherens junctions. Furthermore, the constituents of the undercoat (dense layer underlying the membrane) of adherens junctions were selectively extracted from the fraction rich in junctions. Upon SDS electrophoresis of this extract, 10 polypeptides including vinculin, alpha-actinin, and actin were dominant. The results obtained are discussed with special reference to the molecular organization of the undercoats of cell-to-cell adherens junctions.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.