ArticleExperimental parasitology2014
Refrigeration provides a simple means to synchronize in vitro cultures of Plasmodium falciparum.
Article in Experimental parasitology, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.
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Who cites it
8 citing papers in PubMed.
- Cell cycle checkpoint activity in the malaria parasitemSphere · 2026Article
- Cultivation of Asexual Intraerythrocytic Stages ofPathogens (Basel, Switzerland) · 2023Review
- Impact of Short-Term Storage onAntimicrobial agents and chemotherapy · 2022Article
- Continuous Cultures ofMediterranean journal of hematology and infectious diseases · 2021Article
- Efficient synchronization of Plasmodium knowlesi in vitro cultures using guanidine hydrochloride.Malaria journal · 2019Article
- Validation ofPeerJ · 2019Article
- Case Report: A Case of Severe Cerebral Malaria Managed with Therapeutic Hypothermia and Other Modalities for Brain Edema.The American journal of tropical medicine and hygiene · 2018Article
- Effect of mild medical hypothermia on in vitro growth of Plasmodium falciparum and the activity of anti-malarial drugs.Malaria journal · 2016Article
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12 authors.
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Abstract
Plasmodium falciparum is usually asynchronous during in vitro culture. Highly synchronized cultures of P. falciparum are routinely used in malaria research. Here, we describe a simple synchronization procedure for P. falciparum asexual erythrocytic culture, which involves storage at 4°C for 8-24 h followed by routine culture. When cultures with 27-60% of ring stage were synchronized using this procedure, 70-93% ring stages were obtained after 48 h of culture and relative growth synchrony remained for at least two erythrocytic cycles. To test the suitability of this procedure for subsequent work, drug sensitivity assays were performed using four laboratory strains and four freshly adapted clinical P. falciparum isolates. Parasites synchronized by sorbitol treatment or refrigeration showed similar dose-response curves and comparable IC50 values to four antimalarial drugs. The refrigeration synchronization method is simple, inexpensive, time-saving, and should be especially useful when large numbers of P. falciparum culture are handled.
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