ArticleStructure (London, England : 1993)2014
The structure and assembly mechanism of a novel three-stranded tubulin filament that centers phage DNA.
Article in Structure (London, England : 1993), 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.
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Who cites it
28 citing papers in PubMed, 44 citations in OpenAlex.
- The biology of jumbo phages.Nature communications · 2026Review
- The complex developmental mechanisms of nucleus-forming jumbo phages.Current opinion in microbiology · 2025Review
- The Biology of Nucleus-Forming Jumbo Phages.Annual review of genetics · 2025Review
- Erwinia phage Asesino is a nucleus-forming phage that lacks PhuZ.Scientific reports · 2025Article
- The intricate organizational strategy of nucleus-forming phages.Current opinion in microbiology · 2024Review
- Asesino: a nucleus-forming phage that lacks PhuZ.bioRxiv : the preprint server for biology · 2024Article
- Negative and ambisense RNA virus ribonucleocapsids: more than protective armor.Microbiology and molecular biology reviews : MMBR · 2023Review
- Identifying the core genome of the nucleus-forming bacteriophage family and characterization of Erwinia phage RAY.Cell reports · 2023Article
- The ϕPA3 phage nucleus is enclosed by a self-assembling 2D crystalline lattice.Nature communications · 2023Article
- Replication Compartments of Eukaryotic and Bacterial DNA Viruses: Common Themes Between Different Domains of Host Cells.Annual review of virology · 2022Review
- A cytoskeletal vortex drives phage nucleus rotation during jumbo phage replication in E. coli.Cell reports · 2022Article
- Catching a Walker in the Act-DNA Partitioning by ParA Family of Proteins.Frontiers in microbiology · 2022Review
- Viral speciation through subcellular genetic isolation and virogenesis incompatibility.Nature communications · 2021Article
- Article
- The Phage Nucleus and PhuZ Spindle: Defining Features of the Subcellular Organization and Speciation of Nucleus-Forming Jumbo Phages.Frontiers in microbiology · 2021Review
- Intracellular Organization by Jumbo Bacteriophages.Journal of bacteriology · 2020Review
- Article
- Article
- Prokaryotic cytoskeletons: protein filaments organizing small cells.Nature reviews. Microbiology · 2018Review
- The Phage Nucleus and Tubulin Spindle Are Conserved among Large Pseudomonas Phages.Cell reports · 2017Article
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7 authors at 2 institutions in 1 country.
Funding
Abstract
Tubulins are a universally conserved protein superfamily that carry out diverse biological roles by assembling filaments with very different architectures. The underlying basis of this structural diversity is poorly understood. Here, we determine a 7.1 Å cryo-electron microscopy reconstruction of the bacteriophage-encoded PhuZ filament and provide molecular-level insight into its cooperative assembly mechanism. The PhuZ family of tubulins is required to actively center the phage within infected host cells, facilitating efficient phage replication. Our reconstruction and derived model reveal the first example of a three-stranded tubulin filament. We show that the elongated C-terminal tail simultaneously stabilizes both longitudinal and lateral interactions, which in turn define filament architecture. Identified interaction surfaces are conserved within the PhuZ family, and their mutagenesis compromises polymerization in vitro and in vivo. Combining kinetic modeling of PhuZ filament assembly and structural data, we suggest a common filament structure and assembly mechanism for the PhuZ family of tubulins.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.