ArticleNeuron2014
WIDE AWAKE mediates the circadian timing of sleep onset.
Article in Neuron, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 81 papers.
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Who cites it
81 citing papers in PubMed, 156 citations in OpenAlex.
- Metaplastic sleep regulation inbioRxiv : the preprint server for biology · 2026Article
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- Control of Aedes albopictus populations by silencing of the vesicular GABA transporter (vgat) and the vesicular monoamine transporter (vmat) genes using recombinant Chlorella shRNA.Parasites & vectors · 2025Article
- mWAKE in the Central Amygdala Regulates Fear Learning and Memory.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2025Article
- A Subcircuit in the Suprachiasmatic Nucleus Generates Wakefulness.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2025Article
- Upstream open reading frames dynamically modulate CLOCK protein translation to regulate circadian rhythms and sleep.PLoS biology · 2025Article
- FlyVISTA, an integrated machine learning platform for deep phenotyping of sleep inScience advances · 2025Article
- Enhanced memory despite severe sleep loss in Drosophila insomniac mutants.PLoS biology · 2025Article
- Neuropeptide-Dependent Spike Time Precision and Plasticity in Circadian Output Neurons.The European journal of neuroscience · 2025Article
- Article
- Fly into tranquility: GABA's role in Drosophila sleep.Current opinion in insect science · 2024Review
- FlyVISTA, an Integrated Machine Learning Platform for Deep Phenotyping of Sleep inbioRxiv : the preprint server for biology · 2024Article
- A subclass of evening cells promotes the switch from arousal to sleep at dusk.Current biology : CB · 2024Article
- Tob Regulates the Timing of Sleep Onset at Night inThe Journal of neuroscience : the official journal of the Society for Neuroscience · 2024Article
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- Genome-wide association in Drosophila identifies a role for Piezo and Proc-R in sleep latency.Scientific reports · 2024Article
- Copper enhances aggregational toxicity of mutant huntingtin in a Drosophila model of Huntington's Disease.Biochimica et biophysica acta. Molecular basis of disease · 2024Article
- Orthologs ofSleep advances : a journal of the Sleep Research Society · 2024Article
- Dynamic neuronal instability generates synaptic plasticity and behavior: Insights from Drosophila sleep.Neuroscience research · 2024Review
21 more citing papers are in PubMed but not listed here.
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PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
15 authors at 5 institutions in 1 country.
Funding
Abstract
How the circadian clock regulates the timing of sleep is poorly understood. Here, we identify a Drosophila mutant, wide awake (wake), that exhibits a marked delay in sleep onset at dusk. Loss of WAKE in a set of arousal-promoting clock neurons, the large ventrolateral neurons (l-LNvs), impairs sleep onset. WAKE levels cycle, peaking near dusk, and the expression of WAKE in l-LNvs is Clock dependent. Strikingly, Clock and cycle mutants also exhibit a profound delay in sleep onset, which can be rescued by restoring WAKE expression in LNvs. WAKE interacts with the GABAA receptor Resistant to Dieldrin (RDL), upregulating its levels and promoting its localization to the plasma membrane. In wake mutant l-LNvs, GABA sensitivity is decreased and excitability is increased at dusk. We propose that WAKE acts as a clock output molecule specifically for sleep, inhibiting LNvs at dusk to promote the transition from wake to sleep.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.