ArticleMethods in enzymology2014
Micropattern-guided assembly of overlapping pairs of dynamic microtubules.
Article in Methods in enzymology, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
7 citing papers in PubMed.
- Development of a Microfluidics-Based Approach for Investigating Microtubule Polymer Mechanics.Journal of visualized experiments : JoVE · 2025Article
- Polymer dynamics of Alp7A reveals how two critical concentrations govern assembly of dynamically unstable actin-like proteins.Molecular biology of the cell · 2024Article
- Article
- WH2 and proline-rich domains of WASP-family proteins collaborate to accelerate actin filament elongation.The EMBO journal · 2018Article
- Near-atomic cryo-EM structure of PRC1 bound to the microtubule.Proceedings of the National Academy of Sciences of the United States of America · 2016Article
- Article
- Control of microtubule organization and dynamics: two ends in the limelight.Nature reviews. Molecular cell biology · 2015Review
Corrections and comments
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Authors and funding
6 authors.
Funding
Abstract
Interactions between antiparallel microtubules are essential for the organization of spindles in dividing cells. The ability to form immobilized antiparallel microtubule pairs in vitro, combined with the ability to image them via TIRF microscopy, permits detailed biochemical characterization of microtubule cross-linking proteins and their effects on microtubule dynamics. Here, we describe methods for chemical micropatterning of microtubule seeds on glass surfaces in configurations that specifically promote the formation of antiparallel microtubule overlaps in vitro. We demonstrate that this assay is especially well suited for reconstitution of minimal midzone overlaps stabilized by the antiparallel microtubule cross-linking protein PRC1 and its binding partners. The micropatterning method is suitable for use with a broad range of proteins, and the assay is generally applicable to any microtubule cross-linking protein.
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Registered trials
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