Evidence map›Paper›PMID 24630115›Full record

ArticleMethods in enzymology2014

Measuring kinetochore-microtubule interaction in vitro.

Jonathan W Driver, Andrew F Powers, Krishna K Sarangapani, Sue Biggins, Charles L Asbury

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Methods in enzymology, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
0.6field-weighted citation impact, top 32% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed, 4 citations in OpenAlex.

  1. Article
  2. Article
  3. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors at 2 institutions in 2 countries.

Jonathan W DriverDepartment of Physiology and Biophysics, University of Washington School of Medicine, Seattle, Washington, USA.
Andrew F PowersDepartment of Physiology and Biophysics, University of Washington School of Medicine, Seattle, Washington, USA.
Krishna K SarangapaniDepartment of Physiology and Biophysics, University of Washington School of Medicine, Seattle, Washington, USA.
Sue BigginsDivision of Basic Sciences, Fred Hutchinson Cancer Research Center, Seattle, Washington, USA. Electronic address: sbiggins@fhcrc.org.
Charles L AsburyDepartment of Physiology and Biophysics, University of Washington School of Medicine, Seattle, Washington, USA.
University of Washington · USCape Town HVTN Immunology Laboratory / Hutchinson Centre Research Institute of South Africa · ZA

Funding

Regulation of Chromosome SegregationR01GM064386 · NIGMS · FRED HUTCHINSON CANCER RESEARCH CENTER · PI BIGGINS, SUSAN · 2002 to 2022
$7.2M
Supplement to Biophysical Study of Reconstituted Kinetochore-microtubule AttachmentsR01GM079373 · NIGMS · UNIVERSITY OF WASHINGTON · PI ASBURY, CHARLES · 2006 to 2019
$4.5M
Regulation of Centromeric ChromatinR01GM078069 · NIGMS · FRED HUTCHINSON CANCER RESEARCH CENTER · PI BIGGINS, SUSAN · 2007 to 2015
$2.7M
Genome wide analysis and modeling of signal integration in the Drosophila ovaryR01GM078079 · NIGMS · PRINCETON UNIVERSITY · PI SHVARTSMAN, STANISLAV Y. · 2006 to 2009
$978k
Multicolor TIRF microscope for studying mitotic spindle components at the singleS10RR026406 · NCRR · UNIVERSITY OF WASHINGTON · PI ASBURY, CHARLES · 2010 to 2010
$214k
NCRR NIH HHS S10 RR026406NCRR NIH HHS S10RR026406NIGMS NIH HHS R01 GM064386NIGMS NIH HHS R01GM064386NIGMS NIH HHS R01 GM078069NIGMS NIH HHS R01 GM078079NIGMS NIH HHS R01GM078079NIGMS NIH HHS R01 GM079373NIGMS NIH HHS R01GM079373
6 · The paper itself

Abstract

Many proteins and protein complexes perform sophisticated, regulated functions in vivo. Many of these functions can be recapitulated using in vitro reconstitution, which serves as a means to establish unambiguous cause-effect relationships, for example, between a protein and its phosphorylating kinase. Here, we describe a protocol to purify kinetochores, the protein complexes that attach chromosomes to microtubules during mitosis, and quantitatively assay their microtubule-binding characteristics. Our assays, based on DIC imaging and laser trapping microscopy, are used to measure the attachment of microtubules to kinetochores and the load-bearing capabilities of those attachments. These assays provide a platform for studying kinase disruption of kinetochore-microtubule attachments, which is believed to be critical for correcting erroneous kinetochore-spindle attachments and thereby avoiding chromosome missegregation. The principles of our approach should be extensible to studies of a wide range of force-bearing interactions in biology.

Indexed as

Biomechanical PhenomenaChromosome SegregationEquipment DesignFungal ProteinsKinetochoresMicrotubulesOptical TweezersPhosphotransferasesWeight-BearingYeastsFungal ProteinsPhosphotransferasesChromosomal mis-segregationKinaseLaser trappingMechanoregulationMitosis

Identifiers

PMID24630115
PMCPMC4106247
OpenAlexW236294069

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.