ArticleMethods in enzymology2014
Reconstitution of contractile actomyosin arrays.
Article in Methods in enzymology, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed, 25 citations in OpenAlex.
- State-dependent energy conversion produces degenerate dissipation in active actomyosin networks.bioRxiv : the preprint server for biology · 2026Article
- Topological control of spontaneous failure in active nematic solids.Nature materials · 2026Article
- Actomyosin-Based Nanodevices for Sensing and Actuation: Bridging Biology and Bioengineering.Biosensors · 2025Review
- Cofilin-mediated actin filament network flexibility facilitates 2D to 3D actomyosin shape change.European journal of cell biology · 2024Article
- Membrane tension induces F-actin reorganization and flow in a biomimetic model cortex.Communications biology · 2023Article
- Jamming Transitions in Astrocytes and Glioblastoma Are Induced by Cell Density and Tension.Cells · 2022Article
- F-actin architecture determines constraints on myosin thick filament motion.Nature communications · 2022Article
- Reconstitution of Membrane-tethered Minimal Actin Cortices on Supported Lipid Bilayers.Journal of visualized experiments : JoVE · 2022Article
- Detailed Balance Broken by Catch Bond Kinetics Enables Mechanical-Adaptation in Active Materials.Advanced functional materials · 2021Article
- Intra-bundle contractions enable extensile properties of active actin networks.Scientific reports · 2021Article
- Evolutionarily diverse LIM domain-containing proteins bind stressed actin filaments through a conserved mechanism.Proceedings of the National Academy of Sciences of the United States of America · 2020Article
- Filament Nucleation Tunes Mechanical Memory in Active Polymer Networks.Advanced functional materials · 2019Article
- The Cytoskeleton-A Complex Interacting Meshwork.Cells · 2019Review
- Entropy production rate is maximized in non-contractile actomyosin.Nature communications · 2018Article
- A coevolution-guided model for the rotor of the bacterial flagellar motor.Scientific reports · 2018Article
- A Versatile Framework for Simulating the Dynamic Mechanical Structure of Cytoskeletal Networks.Biophysical journal · 2017Article
- Disordered actomyosin networks are sufficient to produce cooperative and telescopic contractility.Nature communications · 2016Article
- A biomechanical perspective on stress fiber structure and function.Biochimica et biophysica acta · 2015Review
- Reconstituting actomyosin-dependent mechanosensitive protein complexes in vitro.Nature protocols · 2015Article
- Actomyosin sliding is attenuated in contractile biomimetic cortices.Molecular biology of the cell · 2014Article
Corrections and comments
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Authors and funding
3 authors at 3 institutions in 1 country.
Funding
Abstract
Networks and bundles comprised of F-actin and myosin II generate contractile forces used to drive morphogenic processes in both muscle and nonmuscle cells. To elucidate the minimal requirements for contractility and the mechanisms underlying their contractility, model systems reconstituted from a known set of purified proteins in vitro are needed. Here, we describe two experimental protocols our lab has developed to reconstitute 1D bundles and quasi-2D networks of actomyosin that are amenable to quantitative biophysical measurement. These assays have enabled our discovery of the mechanisms of contractility in disordered actomyosin assemblies and of a mechanical feedback between contraction and F-actin severing.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.