ArticleMethods in enzymology2014
Single-molecule studies of actin assembly and disassembly factors.
Article in Methods in enzymology, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed.
- Article
- Functional redundancy and formin-isoform independent localization of tropomyosin paralogs in Saccharomyces cerevisiae.PLoS genetics · 2025Article
- Computational tools for quantifying actin filament numbers, lengths, and bundling.Biology open · 2024Article
- YAP signaling is involved in WDR1-regulated proliferation and migration of non-small-cell lung cancer cells.Experimental biology and medicine (Maywood, N.J.) · 2022Article
- Visualizing Actin and Microtubule Coupling Dynamics In Vitro by Total Internal Reflection Fluorescence (TIRF) Microscopy.Journal of visualized experiments : JoVE · 2022Article
- Single-molecule imaging of IQGAP1 regulating actin filament dynamics.Molecular biology of the cell · 2022Article
- Reconstitution of contractile actomyosin rings in vesicles.Nature communications · 2021Article
- EB1 Directly Regulates APC-Mediated Actin Nucleation.Current biology : CB · 2020Article
- Abp1 promotes Arp2/3 complex-dependent actin nucleation and stabilizes branch junctions by antagonizing GMF.Nature communications · 2018Article
- Single-molecule fluorescence microscopy review: shedding new light on old problems.Bioscience reports · 2017Review
- Molecular stretching modulates mechanosensing pathways.Protein science : a publication of the Protein Society · 2017Review
- Structural Basis of Arp2/3 Complex Inhibition by GMF, Coronin, and Arpin.Journal of molecular biology · 2017Article
- Single-molecule imaging of a three-component ordered actin disassembly mechanism.Nature communications · 2015Article
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Authors and funding
3 authors.
Funding
Abstract
The actin cytoskeleton is very dynamic and highly regulated by multiple associated proteins in vivo. Understanding how this system of proteins functions in the processes of actin network assembly and disassembly requires methods to dissect the mechanisms of activity of individual factors and of multiple factors acting in concert. The advent of single-filament and single-molecule fluorescence imaging methods has provided a powerful new approach to discovering actin-regulatory activities and obtaining direct, quantitative insights into the pathways of molecular interactions that regulate actin network architecture and dynamics. Here we describe techniques for acquisition and analysis of single-molecule data, applied to the novel challenges of studying the filament assembly and disassembly activities of actin-associated proteins in vitro. We discuss the advantages of single-molecule analysis in directly visualizing the order of molecular events, measuring the kinetic rates of filament binding and dissociation, and studying the coordination among multiple factors. The methods described here complement traditional biochemical approaches in elucidating actin-regulatory mechanisms in reconstituted filamentous networks.
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