ArticleMethods in enzymology2014
Biochemical reconstitution of the WAVE regulatory complex.
Article in Methods in enzymology, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 25 citations in OpenAlex.
- Article
- The WAVE regulatory complex interacts with Boc and is required for Shh-mediated axon guidance.iScience · 2024Article
- Structural organization of the retriever-CCC endosomal recycling complex.Nature structural & molecular biology · 2024Article
- Cyfip2 controls the acoustic startle threshold through FMRP, actin polymerization, and GABAbioRxiv : the preprint server for biology · 2024Article
- Article
- Structural Organization of the Retriever-CCC Endosomal Recycling Complex.Research square · 2023Article
- PPP2R1A regulates migration persistence through the NHSL1-containing WAVE Shell Complex.Nature communications · 2023Article
- Structural Organization of the Retriever-CCC Endosomal Recycling Complex.bioRxiv : the preprint server for biology · 2023Article
- Arf GTPase activates the WAVE regulatory complex through a distinct binding site.Science advances · 2022Article
- Structures reveal a key mechanism of WAVE regulatory complex activation by Rac1 GTPase.Nature communications · 2022Article
- Structure of CYRI-B (FAM49B), a key regulator of cellular actin assembly.Acta crystallographica. Section D, Structural biology · 2020Article
- HEM1 deficiency disrupts mTORC2 and F-actin control in inherited immunodysregulatory disease.Science (New York, N.Y.) · 2020Article
- The trimeric coiled-coil HSBP1 protein promotes WASH complex assembly at centrosomes.The EMBO journal · 2018Article
- A Dendritic Guidance Receptor Complex Brings Together Distinct Actin Regulators to Drive Efficient F-Actin Assembly and Branching.Developmental cell · 2018Article
- Article
- Ena/VASP proteins cooperate with the WAVE complex to regulate the actin cytoskeleton.Developmental cell · 2014Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
The WAVE regulatory complex (WRC) is a 400-kDa heteropentameric protein assembly that plays a central role in controlling actin cytoskeletal dynamics in many cellular processes. The WRC acts by integrating diverse cellular cues and stimulating the actin nucleating activity of the Arp2/3 complex at membranes. Biochemical and biophysical studies of the underlying mechanisms of these processes require large amounts of purified WRC. Recent success in recombinant expression, reconstitution, purification, and crystallization of the WRC has greatly advanced our understanding of the inhibition, activation, and membrane recruitment mechanisms of this complex. But many important questions remain to be answered. Here, we summarize and update the methods developed in our laboratory, which allow reliable and flexible production of tens of milligrams of recombinant WRC of crystallographic quality, sufficient for many biochemical and structural studies.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.