ArticleBMC cancer2014
Nanoscale changes in chromatin organization represent the initial steps of tumorigenesis: a transmission electron microscopy study.
Article in BMC cancer, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 50 papers.
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Who cites it
50 citing papers in PubMed, 80 citations in OpenAlex.
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- Early detection of lung cancer using artificial intelligence-enhanced optical nanosensing of chromatin alterations in field carcinogenesis.Scientific reports · 2023Article
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- Epigenome-wide methylation analysis of colorectal carcinoma, adenoma and normal tissue reveals novel biomarkers addressing unmet clinical needs.Clinical epigenetics · 2023Article
- Single cell imaging-based chromatin biomarkers for tumor progression.Scientific reports · 2021Article
- Early Upper Aerodigestive Tract Cancer Detection Using Electron Microscopy to Reveal Chromatin Packing Alterations in Buccal Mucosa Cells.Microscopy and microanalysis : the official journal of Microscopy Society of America, Microbeam Analysis Society, Microscopical Society of Canada · 2021Article
- Origins of subdiffractional contrast in optical coherence tomography.Biomedical optics express · 2021Article
- Qualitative disorder measurements from backscattering spectra through an optical fiber.Biomedical optics express · 2020Article
- Super-resolution imaging reveals the evolution of higher-order chromatin folding in early carcinogenesis.Nature communications · 2020Article
- Kinesin-5 Eg5 is essential for spindle assembly and chromosome alignment of mouse spermatocytes.Cell division · 2020Article
- Disordered chromatin packing regulates phenotypic plasticity.Science advances · 2020Article
- Kinesin-7 CENP-E regulates chromosome alignment and genome stability of spermatogenic cells.Cell death discovery · 2020Article
- A qualitative transcriptional signature for the early diagnosis of colorectal cancer.Cancer science · 2019Article
- Linker histones are fine-scale chromatin architects modulating developmental decisions in Arabidopsis.Genome biology · 2019Article
- Kinesin-14 motor protein KIFC1 participates in DNA synthesis and chromatin maintenance.Cell death & disease · 2019Article
- Chromatin laser imaging reveals abnormal nuclear changes for early cancer detection.Biomedical optics express · 2019Article
- High-resolution microscopy for imaging cancer pathobiology.Current pathobiology reports · 2019Article
- Preservation of cellular nano-architecture by the process of chemical fixation for nanopathology.PloS one · 2019Article
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Authors and funding
10 authors at 4 institutions in 1 country.
Funding
Abstract
backgroundNuclear alterations are a well-known manifestation of cancer. However, little is known about the early, microscopically-undetectable stages of malignant transformation. Based on the phenomenon of field cancerization, the tissue in the field of a tumor can be used to identify and study the initiating events of carcinogenesis. Morphological changes in nuclear organization have been implicated in the field of colorectal cancer (CRC), and we hypothesize that characterization of chromatin alterations in the early stages of CRC will provide insight into cancer progression, as well as serve as a biomarker for early detection, risk stratification and prevention.
methodsFor this study we used transmission electron microscopy (TEM) images of nuclei harboring pre-neoplastic CRC alterations in two models: a carcinogen-treated animal model of early CRC, and microscopically normal-appearing tissue in the field of human CRC. We quantify the chromatin arrangement using approaches with two levels of complexity: 1) binary, where chromatin is separated into areas of dense heterochromatin and loose euchromatin, and 2) grey-scale, where the statistics of continuous mass-density distribution within the nucleus is quantified by its spatial correlation function.
resultsWe established an increase in heterochromatin content and clump size, as well as a loss of its characteristic peripheral positioning in microscopically normal pre-neoplastic cell nuclei. Additionally, the analysis of chromatin density showed that its spatial distribution is altered from a fractal to a stretched exponential.
conclusionsWe characterize quantitatively and qualitatively the nanoscale structural alterations preceding cancer development, which may allow for the establishment of promising new biomarkers for cancer risk stratification and diagnosis. The findings of this study confirm that ultrastructural changes of chromatin in field carcinogenesis represent early neoplastic events leading to the development of well-documented, microscopically detectable hallmarks of cancer.
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