ArticleMicrobial cell factories2014
Genome-scale metabolic network reconstruction of Saccharopolyspora spinosa for spinosad production improvement.
Article in Microbial cell factories, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Who cites it
12 citing papers in PubMed.
- Systematic Engineering of Proteases in Saccharopolyspora Spinosa Reveals Synergistic Enhancement of Spinosad Biosynthesis via Substrate Flux Optimization.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2026Article
- Synthetic Biology of Plants and Microbes for Agriculture, Environment, and Future Applications.Chemical reviews · 2026Review
- Chemical composition and bioinsecticidal activity of bioinputs produced by Saccharopolyspora spinosa.Applied microbiology and biotechnology · 2025Article
- Optimization of spinosad production by Saccharopolysosa spinosa YJY-12 and its application in controlling Eggplant Thrips.Scientific reports · 2025Article
- Developing a robust genome editing tool based on an endogenous type I-B CRISPR-Cas system in Saccharopolyspora spinosa.Science China. Life sciences · 2025Article
- Effects of the pleiotropic regulator DasR on lincomycin production in Streptomyces lincolnensis.Applied microbiology and biotechnology · 2024Article
- Microbial cell factories based on filamentous bacteria, yeasts, and fungi.Microbial cell factories · 2023Review
- Carbon catabolite regulation of secondary metabolite formation, an old but not well-established regulatory system.Microbial biotechnology · 2022Review
- Increasing the heterologous production of spinosad inSynthetic and systems biotechnology · 2021Article
- Comparative transcriptomic analysis of two Saccharopolyspora spinosa strains reveals the relationships between primary metabolism and spinosad production.Scientific reports · 2021Article
- Metabolic network model guided engineering ethylmalonyl-CoA pathway to improve ascomycin production in Streptomyces hygroscopicus var. ascomyceticus.Microbial cell factories · 2017Article
- High Level of Spinosad Production in the Heterologous Host Saccharopolyspora erythraea.Applied and environmental microbiology · 2016Article
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Authors and funding
4 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundSpinosad is a macrolide antibiotic produced by Saccharopolyspora spinosa with aerobic fermentation. However, the wild strain has a low productivity. In this article, a computational guided engineering approach was adopted in order to improve the yield of spinosad in S. spinosa.
resultsFirstly, a genome-scale metabolic network reconstruction (GSMR) for S.spinosa based on its genome information, literature data and experimental data was established. The model was consists of 1,577 reactions, 1,726 metabolites, and 733 enzymes after manually refined. Then, amino acids supplying experiments were performed in order to test the capabilities of the model, and the results showed a high consistency. Subsequently, transhydrogenase (PntAB, EC 1.6.1.2) was chosen as the potential target for spinosad yield improvement based on the in silico metabolic network models. Furthermore, the target gene was manipulated in the parent strain in order to validate the model predictions. At last, shake flask fermentation was carried out which led to spinosad production of 75.32 mg/L, 86.5% higher than the parent strain (40.39 mg/L).
conclusionsResults confirmed the model had a high potential in engineering S. spinosa for spinosad production. It is the first GSMM for S.spinosa, it has significance for a better understanding of the comprehensive metabolism and guiding strain designing of Saccharopolyspora spinosa in the future.
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