ArticleNano letters2014
Dark-field illumination on zero-mode waveguide/microfluidic hybrid chip reveals T4 replisomal protein interactions.
Article in Nano letters, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 31 citations in OpenAlex.
- Strategies for Overcoming the Single-Molecule Concentration Barrier.ACS measurement science au · 2023Review
- Label-Free Physical Techniques and Methodologies for Proteins Detection in Microfluidic Biosensor Structures.Biomedicines · 2022Review
- Zero-mode waveguides and nanopore-based sequencing technologies accelerate single-molecule studies.Biophysics and physicobiology · 2022Article
- Fast interaction dynamics of G-quadruplex and RGG-rich peptides unveiled in zero-mode waveguides.Nucleic acids research · 2021Article
- Correlative nanophotonic approaches to enlighten the nanoscale dynamics of living cell membranes.Biochemical Society transactions · 2021Review
- Zero-mode waveguides can be made better: fluorescence enhancement with rectangular aluminum nanoapertures from the visible to the deep ultraviolet.Nanoscale advances · 2020Article
- Fabrication of Zero Mode Waveguides for High Concentration Single Molecule Microscopy.Journal of visualized experiments : JoVE · 2020Article
- Long-Range Single-Molecule Förster Resonance Energy Transfer between Alexa Dyes in Zero-Mode Waveguides.ACS omega · 2020Article
- Surface passivation of zero-mode waveguide nanostructures: benchmarking protocols and fluorescent labels.Scientific reports · 2020Article
- Zero-Mode Waveguide Nanophotonic Structures for Single Molecule Characterization.Journal of physics D: Applied physics · 2018Article
- Single-Molecule Plasmon Sensing: Current Status and Future Prospects.ACS sensors · 2017Article
- Optofluidic wavelength division multiplexing for single-virus detection.Proceedings of the National Academy of Sciences of the United States of America · 2015Article
- Plasmofluidics: Merging Light and Fluids at the Micro-/Nanoscale.Small (Weinheim an der Bergstrasse, Germany) · 2015Review
- Review
Corrections and comments
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Authors and funding
7 authors at 1 institution in 1 country.
Funding
Abstract
The ability of zero-mode waveguides (ZMWs) to guide light energy into subwavelength-diameter cylindrical nanoapertures has been exploited for single-molecule fluorescence studies of biomolecules at micromolar concentrations, the typical dissociation constants for biomolecular interactions. Although epi-fluorescence microscopy is now adopted for ZMW-based imaging as an alternative to the commercialized ZMW imaging platform, its suitability and performance awaits rigorous examination. Here, we present conical lens-based dark-field fluorescence microscopy in combination with a ZMW/microfluidic chip for single-molecule fluorescence imaging. We demonstrate that compared to epi-illumination, the dark-field configuration displayed diminished background and noise and enhanced signal-to-noise ratios. This signal-to-noise ratio for imaging using the dark-field setup remains essentially unperturbed by the presence of background fluorescent molecules at micromolar concentration. Our design allowed single-molecule FRET studies that revealed weak DNA-protein and protein-protein interactions found with T4 replisomal proteins.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.