ArticleF1000Research2013
Microbiopsy engineered for minimally invasive and suture-free sub-millimetre skin sampling.
Article in F1000Research, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. It is linked to trial NCT06178666 (A Feasibility Study of a Controlled Human Infection Model), which is not on this map. Cited by 17 papers, 1 of them a synthesis that pooled it.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
A Feasibility Study of a Controlled Human Infection Model (CHIM) With Intradermal Bacillus Calmette-Guérin (BCG) Injection in Malawi
Who cites it
17 citing papers in PubMed, 1 synthesis or guideline pooled it.
- Imaging and quantifying drug delivery in skin - Part 2: Fluorescence andvibrational spectroscopic imaging methods.Advanced drug delivery reviews · 2020Pooled it
- Microcore Skin Biopsies: A Novel, Micro-Invasive Skin Biopsy Technique Yielding Full-Thickness Histology.Skin research and technology : official journal of International Society for Bioengineering and the Skin (ISBS) [and] International Society for Digital Imaging of Skin (ISDIS) [and] International Society for Skin Imaging (ISSI) · 2026Article
- Spatially Profiling Trace Cytokine Signatures From Microscopically Derived Skin Samples to Probe Skin Disease Inflammation.Small methods · 2026Article
- Tolerability and Feasibility of Minimally Invasive Canine Skin Sampling: Excellent Tolerability Meets Transcriptomic Challenges.Veterinary dermatology · 2026Article
- Skeletal Muscle Microbiopsies in Children and Adults-Tolerability, Sample Yield, and Analyzability.Muscle & nerve · 2026Article
- Skin-brain dialogue in auto-inflammatory diseases: A new route to biomarkers?Brain, behavior, & immunity - health · 2024Article
- Design and fabrication of customizable microneedles enabled by 3D printing for biomedical applications.Bioactive materials · 2024Review
- Tissue harvest with a laser microbiopsy.Journal of biomedical optics · 2022Article
- Article
- In vivo microscopy as an adjunctive tool to guide detection, diagnosis, and treatment.Journal of biomedical optics · 2022Article
- Cytokine/Chemokine assessment as a complementary diagnostic tool for inflammatory skin diseases.Frontiers in immunology · 2022Article
- AssessingFrontiers in cellular and infection microbiology · 2021Article
- The Future of Precision Prevention for Advanced Melanoma.Frontiers in medicine · 2021Article
- Minimally Invasive Microbiopsies as an Improved Sampling Method for the Diagnosis of Cutaneous Leishmaniasis.Open forum infectious diseases · 2020Article
- A review of microsampling techniques and their social impact.Biomedical microdevices · 2019Review
- 'Mind your Moles' study: protocol of a prospective cohort study of melanocytic naevi.BMJ open · 2018Article
- Minimally invasive microbiopsies: a novel sampling method for identifying asymptomatic, potentially infectious carriers of Leishmania donovani.International journal for parasitology · 2017Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
7 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We describe the development of a sub-millimetre skin punch biopsy device for minimally invasive and suture-free skin sampling for molecular diagnosis and research. Conventional skin punch biopsies range from 2-4 mm in diameter. Local anaesthesia is required and sutures are usually used to close the wound. Our microbiopsy is 0.50 mm wide and 0.20 mm thick. The microbiopsy device is fabricated from three stacked medical grade stainless steel plates tapered to a point and contains a chamber within the centre plate to collect the skin sample. We observed that the application of this device resulted in a 0.21 ± 0.04 mm wide puncture site in volunteer skin using reflectance confocal microscopy. Histological sections from microbiopsied skin revealed 0.22 ± 0.12 mm wide and 0.26 ± 0.09 mm deep puncture sites. Longitudinal observation in microbiopsied volunteers showed that the wound closed within 1 day and was not visible after 7 days. Reflectance confocal microscope images from these same sites showed the formation of a tiny crust that resolved by 3 weeks and was completely undetectable by the naked eye. The design parameters of the device were optimised for molecular analysis using sampled DNA mass as the primary end point in volunteer studies. Finally, total RNA was characterized. The optimised device extracted 5.9 ± 3.4 ng DNA and 9.0 ± 10.1 ng RNA. We foresee that minimally invasive molecular sampling will play an increasingly significant role in diagnostic dermatology and skin research.
Identifiers
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.