ArticleThe Journal of biological chemistry2014
Yeast AMP-activated protein kinase monitors glucose concentration changes and absolute glucose levels.
Article in The Journal of biological chemistry, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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Who cites it
23 citing papers in PubMed, 48 citations in OpenAlex.
- Transcriptional Reprogramming of Lifespan-Associated Genes in Yeast Lacking TOR1.Biochemical genetics · 2026Article
- Context-aware synthetic promoter design using neural networks enables rewiring of eukaryotic transcriptional networks.NPJ systems biology and applications · 2026Article
- Dynamic patterns of gene expression match extracellular signals through push-pull regulation.PLoS genetics · 2025Article
- Microscopy with microfluidics in microgravity using FlightScope.NPJ microgravity · 2025Article
- Quantifying the nuclear localization of fluorescently tagged proteins.Bioinformatics advances · 2025Article
- Phenotypic heterogeneity follows a growth-viability tradeoff in response to amino acid identity.Nature communications · 2024Article
- Exploring carbon source related localization and phosphorylation in the Snf1/Mig1 network using population and single cell-based approaches.Microbial cell (Graz, Austria) · 2024Article
- The yeast 14-3-3 proteins Bmh1 and Bmh2 regulate key signaling pathways.Frontiers in molecular biosciences · 2024Review
- Glucose restriction in Saccharomyces cerevisiae modulates the phosphorylation pattern of the 20S proteasome and increases its activity.Scientific reports · 2023Article
- The Effect of Calorie Restriction on Protein Quality Control in Yeast.Biomolecules · 2023Review
- Using the AKAR3-EV biosensor to assess Sch9p- and PKA-signalling in budding yeast.FEMS yeast research · 2023Article
- The impact of metabolism on the adaptation of organisms to environmental change.Frontiers in cell and developmental biology · 2023Article
- Scalable and flexible inference framework for stochastic dynamic single-cell models.PLoS computational biology · 2022Article
- Metabolic remodeling maintains a reducing environment for rapid activation of the yeast DNA replication checkpoint.The EMBO journal · 2022Article
- Mig1 localization exhibits biphasic behavior which is controlled by both metabolic and regulatory roles of the sugar kinases.Molecular genetics and genomics : MGG · 2020Article
- Fine-Tuning of Energy Levels RegulatesFrontiers in physiology · 2020Article
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- Single-cell study links metabolism with nutrient signaling and reveals sources of variability.BMC systems biology · 2017Article
Corrections and comments
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Authors and funding
8 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Analysis of the time-dependent behavior of a signaling system can provide insight into its dynamic properties. We employed the nucleocytoplasmic shuttling of the transcriptional repressor Mig1 as readout to characterize Snf1-Mig1 dynamics in single yeast cells. Mig1 binds to promoters of target genes and mediates glucose repression. Mig1 is predominantly located in the nucleus when glucose is abundant. Upon glucose depletion, Mig1 is phosphorylated by the yeast AMP-activated kinase Snf1 and exported into the cytoplasm. We used a three-channel microfluidic device to establish a high degree of control over the glucose concentration exposed to cells. Following regimes of glucose up- and downshifts, we observed a very rapid response reaching a new steady state within less than 1 min, different glucose threshold concentrations depending on glucose up- or downshifts, a graded profile with increased cell-to-cell variation at threshold glucose concentrations, and biphasic behavior with a transient translocation of Mig1 upon the shift from high to intermediate glucose concentrations. Fluorescence loss in photobleaching and fluorescence recovery after photobleaching data demonstrate that Mig1 shuttles constantly between the nucleus and cytoplasm, although with different rates, depending on the presence of glucose. Taken together, our data suggest that the Snf1-Mig1 system has the ability to monitor glucose concentration changes as well as absolute glucose levels. The sensitivity over a wide range of glucose levels and different glucose concentration-dependent response profiles are likely determined by the close integration of signaling with the metabolism and may provide for a highly flexible and fast adaptation to an altered nutritional status.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.