ArticlePlant physiology2014
Unraveling vitamin B12-responsive gene regulation in algae.
Article in Plant physiology, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
29 citing papers in PubMed, 70 citations in OpenAlex.
- Multi-omics uncovers nutrient stress-driven interactions in a syntheticISME communications · 2026Article
- A new tool for engineering Phaeodactylum tricornutum: the METE promoter drives both high expression and BThe Plant journal : for cell and molecular biology · 2025Article
- Regulation of nucleus-encoded trans-acting factors allows orthogonal fine-tuning of multiple transgenes in the chloroplast of Chlamydomonas reinhardtii.Plant biotechnology journal · 2025Article
- Conserved cobalamin acquisition protein 1 is essential for vitamin B12 uptake in both Chlamydomonas and Phaeodactylum.Plant physiology · 2024Article
- Scarcity of fixed carbon transfer in a model microbial phototroph-heterotroph interaction.The ISME journal · 2024Article
- Taxonomic, Phylogenomic and Bioactivity Profiling of Novel Phycosphere Bacterium from Model CyanobacteriumMarine drugs · 2024Article
- Zn deficiency disrupts Cu and S homeostasis in Chlamydomonas resulting in over accumulation of Cu and Cysteine.Metallomics : integrated biometal science · 2023Article
- Plant organellar RNA maturation.The Plant cell · 2023Review
- Efficient co-production of EPA and DHA by Schizochytrium sp. via regulation of the polyketide synthase pathway.Communications biology · 2022Article
- Harnessing the Algal Chloroplast for Heterologous Protein Production.Microorganisms · 2022Review
- Assessment of Antioxidant and Anticancer Activities of Microgreen AlgaMolecules (Basel, Switzerland) · 2022Article
- Biocircuits in plants and eukaryotic algae.Frontiers in plant science · 2022Review
- Exploring the Impact of Terminators on Transgene Expression inLife (Basel, Switzerland) · 2021Article
- Microbial and Genetic Resources for Cobalamin (Vitamin B12) Biosynthesis: From Ecosystems to Industrial Biotechnology.International journal of molecular sciences · 2021Review
- Advantages of Heterotrophic Microalgae as a Host for Phytochemicals Production.Frontiers in bioengineering and biotechnology · 2021Review
- Overcoming Algal Vitamin B12 Auxotrophy by Experimental Evolution.Plant physiology · 2020Article
- Responses of a Newly Evolved Auxotroph of Chlamydomonas to BPlant physiology · 2020Article
- Good News for Nuclear Transgene Expression inCells · 2019Review
- The Mars1 kinase confers photoprotection through signaling in the chloroplast unfolded protein response.eLife · 2019Article
- The Synthetic Biology Toolkit for Photosynthetic Microorganisms.Plant physiology · 2019Review
Corrections and comments
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Authors and funding
6 authors at 3 institutions in 3 countries.
Funding
Abstract
Photosynthetic microalgae play a vital role in primary productivity and biogeochemical cycling in both marine and freshwater systems across the globe. However, the growth of these cosmopolitan organisms depends on the bioavailability of nutrients such as vitamins. Approximately one-half of all microalgal species requires vitamin B12 as a growth supplement. The major determinant of algal B12 requirements is defined by the isoform of methionine synthase possessed by an alga, such that the presence of the B12-independent methionine synthase (METE) enables growth without this vitamin. Moreover, the widespread but phylogenetically unrelated distribution of B12 auxotrophy across the algal lineages suggests that the METE gene has been lost multiple times in evolution. Given that METE expression is repressed by the presence of B12, prolonged repression by a reliable source of the vitamin could lead to the accumulation of mutations and eventually gene loss. Here, we probe METE gene regulation by B12 and methionine/folate cycle metabolites in both marine and freshwater microalgal species. In addition, we identify a B12-responsive element of Chlamydomonas reinhardtii METE using a reporter gene approach. We show that complete repression of the reporter occurs via a region spanning -574 to -90 bp upstream of the METE start codon. A proteomics study reveals that two other genes (S-Adenosylhomocysteine hydrolase and Serine hydroxymethyltransferase2) involved in the methionine-folate cycle are also repressed by B12 in C. reinhardtii. The strong repressible nature and high sensitivity of the B12-responsive element has promising biotechnological applications as a cost-effective regulatory gene expression tool.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.