ArticleNucleic acids research2014
Novel functional small RNAs are selectively loaded onto mammalian Ago1.
Article in Nucleic acids research, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed, 30 citations in OpenAlex.
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- A guide to the biogenesis and functions of endogenous small non-coding RNAs in animals.Nature reviews. Molecular cell biology · 2025Review
- PTBP1 protects Y RNA from cleavage leading to its apoptosis-specific degradation.Cell death discovery · 2024Article
- Characterization of Argonaute-containing protein complexes in Leishmania-infected human macrophages.PloS one · 2024Article
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- Lactate Induces Production of the tRNAMolecular therapy : the journal of the American Society of Gene Therapy · 2020Article
- Small RNA fragments derived from multiple RNA classes - the missing element of multi-omics characteristics of the hepatitis C virus cell culture model.BMC genomics · 2017Article
- Interference in transcription of overexpressed genes by promoter-proximal downstream sequences.Scientific reports · 2016Article
- Non-equivalent Roles of AGO1 and AGO2 in mRNA Turnover and Translation of Cyclin D1 mRNA.The Journal of biological chemistry · 2016Article
- Modulation of ALDH5A1 and SLC22A7 by microRNA hsa-miR-29a-3p in human liver cells.Biochemical pharmacology · 2015Article
- MicroRNA hsa-miR-29a-3p modulates CYP2C19 in human liver cells.Biochemical pharmacology · 2015Article
- A legion of potential regulatory sRNAs exists beyond the typical microRNAs microcosm.Nucleic acids research · 2015Article
- Post-transcriptional gene silencing, transcriptional gene silencing and human immunodeficiency virus.World journal of virology · 2015Review
- Assessing the gene regulatory properties of Argonaute-bound small RNAs of diverse genomic origin.Nucleic acids research · 2015Article
- The varied roles of nuclear argonaute-small RNA complexes and avenues for therapy.Molecular therapy. Nucleic acids · 2014Review
- Micro RNA detection in long-term fixed tissue of cortical glutamatergic pyramidal neurons after targeted laser-capture neuroanatomical microdissection.Journal of neuroscience methods · 2014Article
Corrections and comments
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Authors and funding
15 authors at 7 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Argonaute (Ago) proteins function in RNA silencing as components of the RNA-induced silencing complex (RISC). In lower organisms, the small interfering RNA and miRNA pathways diverge due in part to sorting mechanisms that direct distinct small RNA (sRNA) duplexes onto specific Ago-RISCs. However, such sorting mechanisms appear to be lost in mammals. miRNAs appear not to distinguish among Ago1-4. To determine the effect of viral infection on the sorting system, we compared the content of deep-sequenced RNA extracted from immunoprecipitation experiments with the Ago1 and Ago2 proteins using Epstein-Barr virus (EBV)-infected cells. Consistent with previous observations, sequence tags derived from miRNA loci in EBV and humans globally associate in approximately equivalent amounts with Ago1 and Ago2. Interestingly, additional sRNAs, which have not been registered as miRNAs, were associated with Ago1. Among them, some unique sequence tags derived from tandem loci in the human genome associate exclusively with Ago1 but not, or rarely, with Ago2. This is supported by the observation that the expression of the unique sRNAs in the cells is highly dependent on Ago1 proteins. When we knocked down Ago1, the expression of the Ago1-specific sRNAs decreased dramatically. Most importantly, the Ago1-specific sRNAs bound to mRNAs and regulated target genes and were dramatically upregulated, depending on the EBV life cycle. Therefore, even in mammals, the sorting mechanism in the Ago1-4 family is functional. Moreover, the existence of Ago1-specific sRNAs implies vital roles in some aspects of mammalian biology.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.