ArticleAnnals of biomedical engineering2014
Estimating cell concentration in three-dimensional engineered tissues using high frequency quantitative ultrasound.
Article in Annals of biomedical engineering, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
17 citing papers in PubMed.
- Characterizing tendon microstructure using metrics associated with the angular dependence of ultrasound backscatter.The Journal of the Acoustical Society of America · 2026Article
- Article
- Echo intensity and gray-level co-occurrence matrix analysis of soft tissue grafting biomaterials and dental implants: anDento maxillo facial radiology · 2023Article
- Finite Element Modeling of Quantitative Ultrasound Analysis of the Surgical Margin of Breast Tumor.Tomography (Ann Arbor, Mich.) · 2022Article
- Selection and validation of reference gene for RT-qPCR studies in co-culture system of mouse cementoblasts and periodontal ligament cells.BMC research notes · 2022Article
- A novel quantitative and reference-free ultrasound analysis to discriminate different concentrations of bone mineral content.Scientific reports · 2021Article
- Photoacoustic Imaging in Tissue Engineering and Regenerative Medicine.Tissue engineering. Part B, Reviews · 2020Review
- Article
- Non-invasive acoustic fabrication methods to enhance collagen hydrogel bioactivity.Materials research express · 2019Article
- A Multimodal Biomicroscopic System based on High-frequency Acoustic Radiation Force Impulse and Multispectral Imaging Techniques for Tumor Characterization Ex vivo.Scientific reports · 2017Article
- Ultrasound patterning technologies for studying vascular morphogenesis in 3D.Journal of cell science · 2017Article
- Ultrasound Imaging Techniques for Spatiotemporal Characterization of Composition, Microstructure, and Mechanical Properties in Tissue Engineering.Tissue engineering. Part B, Reviews · 2016Review
- Quantitative Ultrasound for Nondestructive Characterization of Engineered Tissues and Biomaterials.Annals of biomedical engineering · 2016Review
- Development of a High-Throughput Ultrasound Technique for the Analysis of Tissue Engineering Constructs.Annals of biomedical engineering · 2016Article
- Non-invasive and Non-destructive Characterization of Tissue Engineered Constructs Using Ultrasound Imaging Technologies: A Review.Annals of biomedical engineering · 2016Review
- Scholte wave generation during single tracking location shear wave elasticity imaging of engineered tissues.The Journal of the Acoustical Society of America · 2015Article
- Noninvasive Quantitative Imaging of Collagen Microstructure in Three-Dimensional Hydrogels Using High-Frequency Ultrasound.Tissue engineering. Part C, Methods · 2015Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors.
Funding
Abstract
Histology and biochemical assays are standard techniques for estimating cell concentration in engineered tissues. However, these techniques are destructive and cannot be used for longitudinal monitoring of engineered tissues during fabrication processes. The goal of this study was to develop high-frequency quantitative ultrasound techniques to nondestructively estimate cell concentration in three-dimensional (3-D) engineered tissue constructs. High-frequency ultrasound backscatter measurements were obtained from cell-embedded, 3-D agarose hydrogels. Two broadband single-element transducers (center frequencies of 30 and 38 MHz) were employed over the frequency range of 13-47 MHz. Agarose gels with cell concentrations ranging from 1 × 10(4) to 1 × 10(6) cells mL(-1) were investigated. The integrated backscatter coefficient (IBC), a quantitative ultrasound spectral parameter, was calculated and used to estimate cell concentration. Accuracy and precision of this technique were analyzed by calculating the percent error and coefficient of variation of cell concentration estimates. The IBC increased linearly with increasing cell concentration. Axial and lateral dimensions of regions of interest that resulted in errors of less than 20% were determined. Images of cell concentration estimates were employed to visualize quantitatively regional differences in cell concentrations. This ultrasound technique provides the capability to rapidly quantify cell concentration within 3-D tissue constructs noninvasively and nondestructively.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.