ArticleInvestigative ophthalmology & visual science2014
Conditional knockout of CTGF affects corneal wound healing.
Article in Investigative ophthalmology & visual science, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
22 citing papers in PubMed, 35 citations in OpenAlex.
- Role of CTGF-LRP1 in impaired healing of cesarean section incisions.Nature communications · 2026Article
- Cold Atmospheric Plasma Enhances TGF-β1, CTGF Protein Expression, and Healing in Full-Thickness Skin Burns: An Animal Study.Biomolecules · 2025Article
- Connective tissue growth factor: Role in trabecular meshwork remodeling and intraocular pressure lowering.Experimental biology and medicine (Maywood, N.J.) · 2023Review
- CCN2/CTGF promotes liver fibrosis through crosstalk with the Slit2/Robo signaling.Journal of cell communication and signaling · 2023Article
- Generation of Conditional KO Mice of CCN2 and Its Function in the Kidney.Methods in molecular biology (Clifton, N.J.) · 2023Article
- Retinal Gene Expression of Selective Genes and Histological Stages of Embryonic and Post-Hatch Chickens (Genes · 2022Article
- The CCN2/CTGF interactome: an approach to understanding the versatility of CCN2/CTGF molecular activities.Journal of cell communication and signaling · 2021Review
- FAK Inhibition Attenuates Corneal Fibroblast Differentiation In Vitro.Biomolecules · 2021Article
- Eyeing the Extracellular Matrix in Vascular Development and Microvascular Diseases and Bridging the Divide between Vascular Mechanics and Function.International journal of molecular sciences · 2020Review
- YAP and TAZ are distinct effectors of corneal myofibroblast transformation.Experimental eye research · 2019Article
- Differential expression of connective tissue growth factor and hepatocyte growth factor in the vitreous of patients with high myopia versus vitreomacular interface disease.BMC ophthalmology · 2019Observational
- Differential Expression of Coding and Long Noncoding RNAs in Keratoconus-Affected Corneas.Investigative ophthalmology & visual science · 2018Article
- Vascular Endothelial Cell-Specific Connective Tissue Growth Factor (CTGF) Is Necessary for Development of Chronic Hypoxia-Induced Pulmonary Hypertension.Frontiers in physiology · 2018Article
- Development of wound healing models to study TGFβ3's effect on SMA.Experimental eye research · 2017Article
- Dual-Phase Iontophoresis for the Delivery of Antisense Oligonucleotides.Nucleic acid therapeutics · 2017Article
- Connective tissue growth factor is not necessary for haze formation in excimer laser wounded mouse corneas.PloS one · 2017Article
- Nanolayered siRNA delivery platforms for local silencing of CTGF reduce cutaneous scar contraction in third-degree burns.Biomaterials · 2016Article
- Characterization of Inhibitor of differentiation (Id) proteins in human cornea.Experimental eye research · 2016Article
- Eyeing the Cyr61/CTGF/NOV (CCN) group of genes in development and diseases: highlights of their structural likenesses and functional dissimilarities.Human genomics · 2015Review
- Connective tissue growth factor and integrin αvβ6: a new pair of regulators critical for ductular reaction and biliary fibrosis in mice.Hepatology (Baltimore, Md.) · 2015Article
Corrections and comments
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Authors and funding
8 authors at 3 institutions in 3 countries.
Funding
Abstract
purposeThis study aimed to elucidate the role of connective tissue growth factor (CTGF) in healthy eyes and wounded corneas of mice and rabbits. Conditional knockout mice were used to determine the role of CTGF in corneal healing.
methodsCTGF expression was determined using transgenic mice carrying CTGF promoter driven-eGFP, quantitative RT-PCR, and immunofluorescent staining. Mice that carried two floxed CTGF alleles and a Cre/ERT2 transgene under the control of human ubiquitin C (ubc) promoter were used to conditionally delete CTGF gene in a tamoxifen-inducible manner. Phototherapeutic keratectomy (PTK) was used to generate an acute corneal wound and corneal re-epithelialization was assessed by fluorescein staining.
resultsConnective tissue growth factor expression was found in multiple ocular tissues with relatively high levels in the corneal endothelium, lens subcapsular epithelium, and in the vasculature of the iris and retina. Wounded corneas responded with an immediate upregulation of CTGF in the epithelium at the wound margin and a sustained CTGF induction during re-epithelialization. At the onset of haze formation, CTGF protein becomes more focused in the basal epithelium. Deletion of the CTGF gene caused a 40% reduction (P < 0.01) in the cornea re-epithelialization rate in knockout mice compared with wild-type mice.
conclusionsConnective tissue growth factor is expressed in the naïve cornea, lens, iris, and retina, and is expressed immediately after epithelial injury. Loss of CTGF impairs efficient re-epithelialization of corneal wounds.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.