ArticlePloS one2014
De novo assembly of Auricularia polytricha transcriptome using Illumina sequencing for gene discovery and SSR marker identification.
Article in PloS one, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers.
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Who cites it
29 citing papers in PubMed, 57 citations in OpenAlex.
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- Transcriptome analysis and development of EST-SSR markers in the mushroom Auricularia heimuer.Scientific reports · 2024Article
- Characterization and development of transcriptome-derived novel EST-SSR markers to assess genetic diversity in3 Biotech · 2023Article
- Lessons on fruiting body morphogenesis from genomes and transcriptomes ofStudies in mycology · 2023Article
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- Comparison of the Nutritional Properties and Transcriptome Profiling Between the Two Different Harvesting Periods ofFrontiers in nutrition · 2021Article
- Genome Survey and Transcriptome Analysis on Mycelia and Primordia ofBioMed research international · 2020Article
- Transcriptional profiling of Auricularia cornea in selenium accumulation.Scientific reports · 2019Article
- Comparative transcriptome analysis reveals relationship of three major domesticated varieties of Auricularia auricula-judae.Scientific reports · 2019Article
- Genomic Analyses Provide Insights Into the Evolutionary History and Genetic Diversity ofFrontiers in microbiology · 2019Article
- Comparative transcriptome analysis of dikaryotic mycelia and mature fruiting bodies in the edible mushroom Lentinula edodes.Scientific reports · 2018Article
- Development of Novel Polymorphic EST-SSR Markers in Bailinggu (Pleurotus tuoliensis) for Crossbreeding.Genes · 2017Article
- Transcriptional profiling provides new insights into the role of nitric oxide in enhancing Ganoderma oregonense resistance to heat stress.Scientific reports · 2017Article
- Article
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Auricularia polytricha (Mont.) Sacc., a type of edible black-brown mushroom with a gelatinous and modality-specific fruiting body, is in high demand in Asia due to its nutritional and medicinal properties. Illumina Solexa sequenceing technology was used to generate very large transcript sequences from the mycelium and the mature fruiting body of A. polytricha for gene discovery and molecular marker development. De novo assembly generated 36,483 ESTs with an N50 length of 636 bp. A total of 28,108 ESTs demonstrated significant hits with known proteins in the nr database, and 94.03% of the annotated ESTs showed the greatest similarity to A. delicata, a related species of A. polytricha. Functional categorization of the Gene Ontology (GO), Clusters of Orthologous Groups (COG) and Kyoto Encyclopedia of Genes and Genomes (KEGG) metabolic pathways revealed the conservation of genes involved in various biological processes in A. polytricha. Gene expression profile analysis indicated that a total of 2,057 ESTs were differentially expressed, including 1,020 ESTs that were up-regulated in the mycelium and 1,037 up-regulated in the fruiting body. Functional enrichment showed that the ESTs associated with biosynthesis, metabolism and assembly of proteins were more active in fruiting body development. The expression patterns of homologous transcription factors indicated that the molecular mechanisms of fruiting body formation and development were not exactly the same as for other agarics. Interestingly, an EST encoding tyrosinase was significantly up-regulated in the fruiting body, indicating that melanins accumulated during the processes of the formation of the black-brown color of the fruiting body in A. polytricha development. In addition, a total of 1,715 potential SSRs were detected in this transcriptome. The transcriptome analysis of A. polytricha provides valuable sequence resources and numerous molecular markers to facilitate further functional genomics studies and genetic researches on this fungus.
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