ArticlePloS one2014
Design and construction of an equibiaxial cell stretching system that is improved for biochemical analysis.
Article in PloS one, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed.
- Deep Learning for Strain Field Customization in Bioreactor with Dielectric Elastomer Actuator Array.Cyborg and bionic systems (Washington, D.C.) · 2024Article
- Systems Biology Analysis of Temporal Dynamics That Govern Endothelial Response to Cyclic Stretch.Biomolecules · 2022Review
- A Fully Integrated Arduino-Based System for the Application of Stretching Stimuli to Living Cells and Their Time-Lapse Observation: A Do-It-Yourself Biology Approach.Annals of biomedical engineering · 2021Article
- Review
- Expression of HIF‑1α in cycling stretch‑induced osteogenic differentiation of bone mesenchymal stem cells.Molecular medicine reports · 2019Article
- Tissue Regeneration from Mechanical Stretching of Cell-Cell Adhesion.Tissue engineering. Part C, Methods · 2019Review
- Cyclic Stretching of Fibrotic Microtissue Array for Evaluation of Anti-Fibrosis Drugs.Cellular and molecular bioengineering · 2019Article
- Characterization of 3D Printed Stretching Devices for Imaging Force Transmission in Live-Cells.Cellular and molecular bioengineering · 2019Article
- Review
- Live cell imaging reveals focal adhesions mechanoresponses in mammary epithelial cells under sustained equibiaxial stress.Scientific reports · 2018Article
- Biochemical analysis of force-sensitive responses using a large-scale cell stretch device.Cell adhesion & migration · 2017Article
- Computationally Informed Design of a Multi-Axial Actuated Microfluidic Chip Device.Scientific reports · 2017Article
- Biophysical Tools to Study Cellular Mechanotransduction.Bioengineering (Basel, Switzerland) · 2017Review
- Stretch Injury of Human Induced Pluripotent Stem Cell Derived Neurons in a 96 Well Format.Scientific reports · 2016Article
Corrections and comments
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Authors and funding
6 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We describe the design and validation of an equibiaxial stretching device in which cells are confined to regions of homogeneous strain. Using this device, we seek to overcome a significant limitation of existing equibiaxial stretching devices, in which strains are not homogeneous over the entire region of cell culture. We cast PDMS in a mold to produce a membrane with a cylindrical wall incorporated in the center, which was used to confine cell monolayers to the central membrane region subjected to homogeneous equibiaxial strain. We demonstrated that the presence of the wall to hold the culture medium did not affect strain homogeneity over the majority of the culture surface and also showed that cells adhered well onto the PDMS membranes. We used our device in cyclic strain experiments and demonstrated strain-dependent changes in extracellular signal-regulated kinase (ERK) and tyrosine phosphorylation upon cell stretching. Furthermore, we examined cell responses to very small magnitudes of strain ranging from 1% to 6% and were able to observe a graduated increase in ERK phosphorylation in response to these strains. Collectively, we were able to study cellular biochemical response with a high degree of accuracy and sensitivity to fine changes in substrate strain. Because we have designed our device along the lines of existing equibiaxial stretching technologies, we believe that our innovations can be incorporated into existing systems. This device would provide a useful addition to the set of tools applied for in vitro studies of cell mechanobiology.
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