Evidence map›Paper›PMID 24626163›Full record

ArticlePloS one2014

Impact of tumour epithelial subtype on circulating microRNAs in breast cancer patients.

Peadar S Waters, Roisin M Dwyer, Cathy Brougham, Claire L Glynn, Deirdre Wall, Peter Hyland, Maria Duignan, Mark McLoughlin, John Newell, Michael J Kerin

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in PloS one, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.

0numbers the graph read from it
0cells of the map it votes in
8citing papers in PubMed
0.8field-weighted citation impact, top 29% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

8 citing papers in PubMed, 15 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors at 1 institution in 1 country.

Peadar S WatersDiscipline of Surgery, School of Medicine, National University of Ireland Galway, Galway, Ireland.
Roisin M DwyerDiscipline of Surgery, School of Medicine, National University of Ireland Galway, Galway, Ireland.
Cathy BroughamDiscipline of Surgery, School of Medicine, National University of Ireland Galway, Galway, Ireland.
Claire L GlynnDiscipline of Surgery, School of Medicine, National University of Ireland Galway, Galway, Ireland.
Deirdre WallHRB Clinical Research Facility and School of Mathematics, Statistics and Applied Mathematics, National University of Galway, Galway, Ireland.
Peter HylandDiscipline of Surgery, School of Medicine, National University of Ireland Galway, Galway, Ireland.
Maria DuignanDiscipline of Surgery, School of Medicine, National University of Ireland Galway, Galway, Ireland.
Mark McLoughlinDiscipline of Surgery, School of Medicine, National University of Ireland Galway, Galway, Ireland.
John NewellHRB Clinical Research Facility and School of Mathematics, Statistics and Applied Mathematics, National University of Galway, Galway, Ireland.
Michael J KerinDiscipline of Surgery, School of Medicine, National University of Ireland Galway, Galway, Ireland.
Ollscoil na Gaillimhe – University of Galway · IE

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

While a range of miRNAs have been shown to be dysregulated in the circulation of patients with breast cancer, little is known about the relationship between circulating levels and tumour characteristics. The aim of this study was to analyse alterations in circulating miRNA expression during tumour progression in a murine model of breast cancer, and to detemine the clinical relevance of identified miRNAs at both tissue and circulating level in patient samples. Athymic nude mice received a subcutaneous or mammary fat pad injection of MDA-MB-231 cells. Blood sampling was performed at weeks 1, 3 and 6 following tumour induction, and microRNA extracted. MicroRNA microArray analysis was performed comparing samples harvested at week 1 to those collected at week 6 from the same animals. Significantly altered miRNAs were validated across all murine samples by RQ-PCR (n = 45). Three miRNAs of interest were then quantified in the circulation(n = 166) and tissue (n = 100) of breast cancer patients and healthy control individuals. MicroArray-based analysis of murine blood samples revealed levels of 77 circulating microRNAs to be changed during disease progression, with 44 demonstrating changes >2-fold. Validation across all samples revealed miR-138 to be significantly elevated in the circulation of animals during disease development, with miR-191 and miR-106a levels significantly decreased. Analysis of patient tissue and blood samples revealed miR-138 to be significantly up-regulated in the circulation of patients with breast cancer, with no change observed in the tissue setting. While not significantly changed overall in breast cancer patients compared to controls, circulating miR-106a and miR-191 were significantly decreased in patients with basal breast cancer. In tissue, both miRNAs were significantly elevated in breast cancer compared to normal breast tissue. The data demonstrates an impact of tumour epithelial subtype on circulating levels of miRNAs, and highlights divergent miRNA profiles between tissue and blood samples from breast cancer patients.

Indexed as

AnimalsBiomarkers, TumorBreast NeoplasmsCarcinomaCell Line, TumorDisease ProgressionFemaleGene Expression Regulation, NeoplasticHumansMiceMice, NudeMicroRNAsMiddle AgedOligonucleotide Array Sequence AnalysisPolymerase Chain ReactionBiomarkers, TumorMicroRNAs

Identifiers

PMID24626163
PMCPMC3953120
OpenAlexW2061098867

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.