ArticleScientific reports2014
Quantitative proteomics of Xenopus laevis embryos: expression kinetics of nearly 4000 proteins during early development.
Article in Scientific reports, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 66 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
66 citing papers in PubMed, 103 citations in OpenAlex.
- In-Cell Proteomics Enables High-Resolution Spatial and Temporal Mapping of Early Xenopus tropicalis Embryos.Molecular & cellular proteomics : MCP · 2026Article
- Quantitative proteomics reveals the dynamic proteome landscape of zebrafish embryos during the maternal-to-zygotic transition.iScience · 2024Article
- Direct Activation of Nucleobases with Small Molecules for the Conditional Control of Antisense Function.Angewandte Chemie (International ed. in English) · 2024Article
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- Expanding the Genetic Code ofACS chemical biology · 2024Article
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- Miniprep assisted proteomics (MAP) for rapid proteomics sample preparation.Analytical methods : advancing methods and applications · 2023Article
- Separation methods in single-cell proteomics: RPLC or CE?International journal of mass spectrometry · 2022Article
- Proteo-genetic analysis reveals clear hierarchy of ESX-1 secretion inProceedings of the National Academy of Sciences of the United States of America · 2022Article
- Applications of Tandem Mass Spectrometry (MS/MS) in Protein Analysis for Biomedical Research.Molecules (Basel, Switzerland) · 2022Review
- Review
- Differential proteome response to H5N1 highly pathogenic avian influenza (HPAI) viruses infection in duck.Frontiers in immunology · 2022Article
- Comparative antler proteome of sika deer from different developmental stages.Scientific reports · 2021Article
- A temporally resolved transcriptome for developing "Keller" explants of the Xenopus laevis dorsal marginal zone.Developmental dynamics : an official publication of the American Association of Anatomists · 2021Article
- Spatially Resolved Mass Spectrometry at the Single Cell: Recent Innovations in Proteomics and Metabolomics.Journal of the American Society for Mass Spectrometry · 2021Review
- [Methods and applications of single-cell proteomics analysis based on mass spectrometry].Se pu = Chinese journal of chromatography · 2021Review
- Mass spectrometry based proteomics for developmental neurobiology in the amphibian Xenopus laevis.Current topics in developmental biology · 2021Review
- The neglected part of early embryonic development: maternal protein degradation.Cellular and molecular life sciences : CMLS · 2020Review
- The power of amphibians to elucidate mechanisms of size control and scaling.Experimental cell research · 2020Review
6 more citing papers are in PubMed but not listed here.
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
Abstract
While there is a rich literature on transcription dynamics during the development of many organisms, protein data is limited. We used iTRAQ isotopic labeling and mass spectrometry to generate the largest developmental proteomic dataset for any animal. Expression dynamics of nearly 4,000 proteins of Xenopus laevis was generated from fertilized egg to neurula embryo. Expression clusters into groups. The cluster profiles accurately reflect the major events that mark changes in gene expression patterns during early Xenopus development. We observed decline in the expression of ten DNA replication factors after the midblastula transition (MBT), including a marked decline of the licensing factor XCdc6. Ectopic expression of XCdc6 leads to apoptosis; temporal changes in this protein are critical for proper development. Measurement of expression in single embryos provided no evidence for significant protein heterogeneity between embryos at the same stage of development.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.