ArticlePLoS genetics2014
The ABI4-induced Arabidopsis ANAC060 transcription factor attenuates ABA signaling and renders seedlings sugar insensitive when present in the nucleus.
Article in PLoS genetics, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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Who cites it
24 citing papers in PubMed, 60 citations in OpenAlex.
- Article
- Physiological and transcriptome analysis of changes in endogenous hormone and sugar content during the formation of tender asparagus stems.BMC plant biology · 2024Article
- Transcriptional Control of Seed Life: New Insights into the Role of the NAC Family.International journal of molecular sciences · 2024Review
- Should I stay or should I go? Trafficking of plant extra-nuclear transcription factors.The Plant cell · 2024Review
- Bet-hedging and variability in plant development: seed germination and beyond.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2024Review
- Gene Cloning and Characterization of Transcription FactorInternational journal of molecular sciences · 2023Article
- Rice lipid transfer protein, OsLTPL23, controls seed germination by regulating starch-sugar conversion and ABA homeostasis.Frontiers in genetics · 2023Article
- Article
- TOR kinase, a GPS in the complex nutrient and hormonal signaling networks to guide plant growth and development.Journal of experimental botany · 2022Review
- The membrane associated NAC transcription factors ANAC060 and ANAC040 are functionally redundant in the inhibition of seed dormancy in Arabidopsis thaliana.Journal of experimental botany · 2022Article
- Genome-wide association study reveals a NAC transcription factor TaNAC074 linked to pre-harvest sprouting tolerance in wheat.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2022Article
- Updated role of ABA in seed maturation, dormancy, and germination.Journal of advanced research · 2022Review
- Proteolytic Activation of Plant Membrane-Bound Transcription Factors.Frontiers in plant science · 2022Review
- Redox feedback regulation of ANAC089 signaling alters seed germination and stress response.Cell reports · 2021Article
- Article
- Multifaceted Signaling Networks Mediated by Abscisic Acid Insensitive 4.Plant communications · 2020Review
- The Arabidopsis Nodulin Homeobox Factor AtNDX Interacts with AtRING1A/B and Negatively Regulates Abscisic Acid Signaling.The Plant cell · 2020Article
- Trait analysis reveals DOG1 determines initial depth of seed dormancy, but not changes during dormancy cycling that result in seedling emergence timing.The New phytologist · 2020Article
- RNA-Seq reveals novel genes and pathways associated with hypoxia duration and tolerance in tomato root.Scientific reports · 2020Article
- The Sugar-Signaling Hub: Overview of Regulators and Interaction with the Hormonal and Metabolic Network.International journal of molecular sciences · 2018Review
Corrections and comments
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Authors and funding
11 authors at 5 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Seedling establishment is inhibited on media containing high levels (∼ 6%) of glucose or fructose. Genetic loci that overcome the inhibition of seedling growth on high sugar have been identified using natural variation analysis and mutant selection, providing insight into sugar signaling pathways. In this study, a quantitative trait locus (QTL) analysis was performed for seedling sensitivity to high sugar in a Col/C24 F2 population of Arabidopsis thaliana. A glucose and fructose-sensing QTL, GSQ11, was mapped through selective genotyping and confirmed in near-isogenic lines in both Col and C24 backgrounds. Allelism tests and transgenic complementation showed that GSQ11 lies within the ANAC060 gene. The Col ANAC060 allele confers sugar insensitivity and was dominant over the sugar-sensitive C24 allele. Genomic and mRNA analyses showed that a single-nucleotide polymorphism (SNP) in Col ANAC060 affects the splicing patterns of ANAC060 such that 20 additional nucleotides are present in the mRNA. The insertion created a stop codon, resulting in a truncated ANAC60 protein lacking the transmembrane domain (TMD) that is present in the C24 ANAC060 protein. The absence of the TMD results in the nuclear localization of ANAC060. The short version of the ANAC060 protein is found in ∼ 12% of natural Arabidopsis accessions. Glucose induces GSQ11/ANAC060 expression in a process that requires abscisic acid (ABA) signaling. Chromatin immunoprecipitation-qPCR and transient expression analysis showed that ABI4 directly binds to the GSQ11/ANAC060 promoter to activate transcription. Interestingly, Col ANAC060 reduced ABA sensitivity and Glc-induced ABA accumulation, and ABI4 expression was also reduced in Col ANAC060 lines. Thus, the sugar-ABA signaling cascade induces ANAC060 expression, but the truncated Col ANAC060 protein attenuates ABA induction and ABA signaling. This negative feedback from nuclear ANAC060 on ABA signaling results in sugar insensitivity.
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