ArticleNature protocols2014
Tracking the sources of blood meals of parasitic arthropods using shotgun proteomics and unidentified tandem mass spectral libraries.
Article in Nature protocols, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.
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Who cites it
10 citing papers in PubMed.
- Shotgun Proteomics Protocol for Insects.Methods in molecular biology (Clifton, N.J.) · 2025Article
- Rewinding the Molecular Clock: Looking at Pioneering Molecular Phylogenetics Experiments in the Light of Proteomics.Journal of proteome research · 2021Article
- Review
- Interactions Between Ticks and Lyme Disease Spirochetes.Current issues in molecular biology · 2021Article
- A novel MALDI-TOF MS-based method for blood meal identification in insect vectors: A proof of concept study on phlebotomine sand flies.PLoS neglected tropical diseases · 2019Article
- Blood meal acquisition enhances arbovirus replication in mosquitoes through activation of the GABAergic system.Nature communications · 2017Article
- How do biting disease vectors behaviourally respond to host availability?Parasites & vectors · 2016Article
- Top-down-assisted bottom-up method for homologous protein sequencing: hemoglobin from 33 bird species.Journal of the American Society for Mass Spectrometry · 2015Article
- Reliability of molecular host-identification methods for ticks: an experimental in vitro study with Ixodes ricinus.Parasites & vectors · 2015Article
- Comparison of Vertebrate Cytochrome b and Prepronociceptin for Blood Meal Analyses in Culicoides.Frontiers in veterinary science · 2015Article
Corrections and comments
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Authors and funding
4 authors.
Funding
Abstract
Identifying the species on which hematophagous arthropods feed is crucial for studying the factors that affect pathogen distributions and that can aid public health. Here we describe a protocol to identify the species a parasitic arthropod has previously fed upon by identifying the source of the remnants of a previous blood meal via shotgun proteomics and spectral matching. The protocol is a nontargeted approach that uses the entire detected blood proteome for source identification; it does not require a priori knowledge of genome or protein sequences. Instead, reference spectral libraries are compiled from the blood of multiple host species by using SpectraST, which takes ∼4 d; the identification of the species from which a previous blood meal of a hematophagous arthropod was taken is achieved with spectral matching against the reference spectral libraries, which takes approximately another 4 d. This method is robust against random degradation of the blood meal and can identify unknown blood remnants months after the feeding event.
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