ArticleScientific reports2014
Interactome analysis of AMP-activated protein kinase (AMPK)-α1 and -β1 in INS-1 pancreatic beta-cells by affinity purification-mass spectrometry.
Article in Scientific reports, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed, 40 citations in OpenAlex.
- Multilevel metabolic adaptation to exercise training.Communications medicine · 2026Article
- PCSK9 stimulates Syk, PKCδ, and NF-κB, leading to atherosclerosis progression independently of LDL receptor.Nature communications · 2024Article
- Interaction between DLC-1 and SAO-1 facilitates CED-4 translocation during apoptosis in the Caenorhabditis elegans germline.Cell death discovery · 2022Article
- AMPK controls the axonal regenerative ability of dorsal root ganglia sensory neurons after spinal cord injury.Nature metabolism · 2020Article
- AMPK Profiling in Rodent and Human Pancreatic Beta-Cells under Nutrient-Rich Metabolic Stress.International journal of molecular sciences · 2020Article
- AMPK Interactome Reveals New Function in Non-homologous End Joining DNA Repair.Molecular & cellular proteomics : MCP · 2020Article
- Mechanisms involved in AMPK-mediated deposition of tight junction components to the plasma membrane.American journal of physiology. Cell physiology · 2020Article
- PINCH-1 interacts with myoferlin to promote breast cancer progression and metastasis.Oncogene · 2020Article
- Troponin destabilization impairs sarcomere-cytoskeleton interactions in iPSC-derived cardiomyocytes from dilated cardiomyopathy patients.Scientific reports · 2020Article
- α-Parvin promotes breast cancer progression and metastasis through interaction with G3BP2 and regulation of TWIST1 signaling.Oncogene · 2019Article
- FoxK1 and FoxK2 in insulin regulation of cellular and mitochondrial metabolism.Nature communications · 2019Article
- ΔNp63α Suppresses TGFB2 Expression and RHOA Activity to Drive Cell Proliferation in Squamous Cell Carcinomas.Cell reports · 2018Article
- Kindlin-2 Association with Rho GDP-Dissociation InhibitorJournal of the American Society of Nephrology : JASN · 2017Article
- Protease-Inhibitor Interaction Predictions: Lessons on the Complexity of Protein-Protein Interactions.Molecular & cellular proteomics : MCP · 2017Article
- Review
- AMPK activity regulates trafficking of mitochondria to the leading edge during cell migration and matrix invasion.Molecular biology of the cell · 2016Article
- Mild Glucose Starvation Induces KDM2A-Mediated H3K36me2 Demethylation through AMPK To Reduce rRNA Transcription and Cell Proliferation.Molecular and cellular biology · 2015Article
- Oestrogen receptors interact with the α-catalytic subunit of AMP-activated protein kinase.Bioscience reports · 2015Article
- Nitric oxide stress and activation of AMP-activated protein kinase impair β-cell sarcoendoplasmic reticulum calcium ATPase 2b activity and protein stability.Cell death & disease · 2015Article
- The biology of IQGAP proteins: beyond the cytoskeleton.EMBO reports · 2015Review
Corrections and comments
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The heterotrimeric enzyme AMP-activated protein kinase (AMPK) is a major metabolic factor that regulates the homeostasis of cellular energy. In particular, AMPK mediates the insulin resistance that is associated with type 2 diabetes. Generally, cellular processes require tight regulation of protein kinases, which is effected through their formation of complex with other proteins and substrates. Despite their critical function in regulation and pathogenesis, there are limited data on the interaction of protein kinases. To identify proteins that interact with AMPK, we performed large-scale affinity purification (AP)-mass spectrometry (MS) of the AMPK-α1 and -β1 subunits. Through a comprehensive analysis, using a combination of immunoprecipitaion and ion trap mass spectrometry, we identified 381 unique proteins in the AMPKα/β interactomes: 325 partners of AMPK-α1 and 243 for AMPK-β1. Further, we identified 196 novel protein-protein interactions with AMPK-α1 and AMPK-β1. Notably, in our bioinformatics analysis, the novel interaction partners mediated functions that are related to the regulation of actin organization. Specifically, several such proteins were linked to pancreatic beta-cell functions, including glucose-stimulated insulin secretion, beta-cell development, beta-cell differentiation, and cell-cell communication.
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