ArticleInfection and immunity1989
Identification of wall-specific antigens synthesized during germ tube formation by Candida albicans.
Article in Infection and immunity, 1989. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 52 papers.
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Who cites it
52 citing papers in PubMed, 145 citations in OpenAlex.
- Preparation of combined extract of cell wall and cytosol antigens of Candida albicans for immunoblot analysis.Journal of clinical laboratory analysis · 2007Article
- ABG1, a novel and essential Candida albicans gene encoding a vacuolar protein involved in cytokinesis and hyphal branching.Eukaryotic cell · 2005Article
- Purification and partial characterization of a Paracoccidioides brasiliensis protein with capacity to bind to extracellular matrix proteins.Infection and immunity · 2005Article
- Differentiation of Candida albicans and Candida dubliniensis by using recombinant human antibody single-chain variable fragments specific for hyphae.Journal of clinical microbiology · 2003Article
- Low levels of antigenic variability in fluconazole-susceptible and -resistant Candida albicans isolates from human immunodeficiency virus-infected patients with oropharyngeal candidiasis.Clinical and diagnostic laboratory immunology · 1999Article
- The cell wall-associated glyceraldehyde-3-phosphate dehydrogenase of Candida albicans is also a fibronectin and laminin binding protein.Infection and immunity · 1998Article
- Detection of antibodies to Candida albicans germ tubes during experimental infections by different Candida species.Clinical and diagnostic laboratory immunology · 1998Article
- Cell wall and secreted proteins of Candida albicans: identification, function, and expression.Microbiology and molecular biology reviews : MMBR · 1998Review
- Serologic response to cell wall mannoproteins and proteins of Candida albicans.Clinical microbiology reviews · 1998Review
- Hemin induces germ tube formation in Candida albicans.Infection and immunity · 1997Article
- The glycolytic enzyme glyceraldehyde-3-phosphate dehydrogenase of Candida albicans is a surface antigen.Journal of bacteriology · 1997Article
- Cloning and characterization of CSP37, a novel gene encoding a putative membrane protein of Candida albicans.Journal of bacteriology · 1997Article
- Binding of extracellular matrix proteins to Aspergillus fumigatus conidia.Infection and immunity · 1996Article
- Evidence for presence in the cell wall of Candida albicans of a protein related to the hsp70 family.Infection and immunity · 1996Article
- Purification and biochemical characterization of a 65-kilodalton mannoprotein (MP65), a main target of anti-Candida cell-mediated immune responses in humans.Infection and immunity · 1996Article
- Binding of human fibronectin to Aspergillus fumigatus conidia.Infection and immunity · 1996Article
- Common and form-specific cell wall antigens of Candida albicans as released by chemical and enzymatic treatments.Mycopathologia · 1996Article
- Preliminary characterization of the material released to the culture medium by Candida albicans yeast and mycelial cells.Antonie van Leeuwenhoek · 1995Article
- Involvement of transglutaminase in the formation of covalent cross-links in the cell wall of Candida albicans.Archives of microbiology · 1995Article
- Evidence for the presence of collagenous domains in Candida albicans cell surface proteins.Infection and immunity · 1995Article
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Walls of the two cellular forms (blastoconidia and mycelia) of Candida albicans ATCC 26555 were obtained from cells metabolically labeled (6-h pulse) with 14C-protein hydrolysate and [3H]threonine. Walls were purified by thorough washings with buffered and sodium dodecyl sulfate solutions and digested with Zymolyase 20T. The enzymatic treatment released four major high-molecular-weight mannoproteins (HMWM), with apparent molecular masses of 650, 500, 340, and 200 kilodaltons (HMWM-650, HMWM-500, HMWM-340, and HMWM-200, respectively), from yeast cells, whereas two high-molecular-mass mannoproteins (HMWM-260 and HMWM-180) were solubilized from mycelial cells. Some additional minor low-molecular-weight species were also detected in the enzymatic digests of walls from both types of cell. Single and dual pulse-chase experiments indicated that the HMWM-260 and HMWM-180 species reflect de novo synthesis of new proteins specific for the mycelia and do not represent a topological rearrangement of blastoconidium wall components. Monoclonal antibodies were raised against the HMWM-260 species (quantitatively the predominant component in the mycelial walls), and polyclonal rabbit antibodies were obtained against yeast or mycelial cell walls. Anti-mycelial cell wall polyclonal antibodies were adsorbed to whole killed blastoconidia to remove antibodies against common blastoconidium and mycelial wall antigens. Titration by enzyme-linked immunosorbent assay revealed that the monoclonal antibodies could recognize an epitope of the protein moiety of the HMWM-260 mannoprotein. Immunoblotting and immunofluorescence techniques using these monoclonal and polyclonal antibodies confirmed that the HMWM-260 and HMWM-180 species are specific components of the envelope of the mycelial cell walls.
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