ArticleBiotechnology for biofuels2014
Differences in the adsorption of enzymes onto lignins from diverse types of lignocellulosic biomass and the underlying mechanism.
Article in Biotechnology for biofuels, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 31 papers.
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Who cites it
31 citing papers in PubMed, 207 citations in OpenAlex.
- Insights into Molecular Mechanism of Secondary Xylem Rapid Growth inPlants (Basel, Switzerland) · 2025Article
- Deciphering the Impact of Lignin on Anaerobic Digestion: Focus on Inhibition Mechanisms and Methods for Alleviating Inhibition.ACS omega · 2024Review
- Mild γ-Butyrolactone/Water Pretreatment for Highly Efficient Sugar Production from Corn Stover.Applied biochemistry and biotechnology · 2024Article
- Bioinspired Polymer Assemblies of Plant Cell Walls for Measuring Protein-Carbohydrate Interactions by FRAP.Methods in molecular biology (Clifton, N.J.) · 2023Article
- Comparative Evaluation of Adsorption of Major Enzymes in a Cellulase Cocktail Obtained fromPolymers · 2022Article
- Recombinant Family 1 Carbohydrate-Binding Modules Derived From Fungal Cellulase Enhance Enzymatic Degradation of Lignocellulose as Novel Effective Accessory Protein.Frontiers in microbiology · 2022Article
- Quantifying the inhibitory impact of soluble phenolics on anaerobic carbon mineralization in a thawing permafrost peatland.PloS one · 2022Article
- Recent advances in understanding the effects of lignin structural characteristics on enzymatic hydrolysis.Biotechnology for biofuels · 2021Review
- CRISPR-Knockout ofInternational journal of molecular sciences · 2021Article
- Enzymatic Pretreatment with Laccases fromInternational journal of molecular sciences · 2021Article
- Cellulose induced protein 1 (Cip1) from Trichoderma reesei enhances the enzymatic hydrolysis of pretreated lignocellulose.Microbial cell factories · 2021Article
- Non-productive binding of cellobiohydrolase i investigated by surface plasmon resonance spectroscopy.Cellulose (London, England) · 2021Article
- Effects of oligolignol sizes and binding modes on a GH11 xylanase inhibition revealed by molecular modeling techniques.Journal of molecular modeling · 2020Article
- Effect of cellulolytic enzyme binding on lignin isolated from alkali and acid pretreated switchgrass on enzymatic hydrolysis.3 Biotech · 2020Article
- NMR elucidation of nonproductive binding sites of lignin models with carbohydrate-binding module of cellobiohydrolase I.Biotechnology for biofuels · 2020Article
- Substrate-Related Factors Affecting Cellulosome-Induced Hydrolysis for Lignocellulose Valorization.International journal of molecular sciences · 2019Review
- NMR Analysis on Molecular Interaction of Lignin with Amino Acid Residues of Carbohydrate-Binding Module from Trichoderma reesei Cel7A.Scientific reports · 2019Article
- Sequential fractionation of the lignocellulosic components in hardwood based on steam explosion and hydrotropic extraction.Biotechnology for biofuels · 2019Article
- Lignocellulosic Biomass: Understanding Recalcitrance and Predicting Hydrolysis.Frontiers in chemistry · 2019Review
- A structured understanding of cellobiohydrolase I binding to poplar lignin fractions after dilute acid pretreatment.Biotechnology for biofuels · 2018Article
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Authors and funding
7 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundNon-productive cellulase adsorption onto lignin has always been deemed to negatively affect the enzymatic hydrolysis of lignocellulosic feedstocks. Therefore, understanding enzyme-lignin interactions is essential for the development of enzyme mixtures, the processes of lignocellulose hydrolysis, and the genetic modification of lignocellulosic biomass and enzymes. In this work, we examined the properties of six lignins from diverse types of lignocellulosic biomass (aspen, pine, corn stover, kenaf, and two Arabidopsis lines, wild-type and SALK mutant of fah1) to determine the mechanism of differences in their adsorption of enzymes.
resultsWe found that lignin sources affected enzyme adsorption using structural features, such as functional groups and lignin composition. Guaiacyl (G) lignin had a higher adsorption capacity on enzymes than syringyl (S) lignin. The low S/G ratio and high uniform lignin fragment size had good correlations with high adsorption capacity. A higher content of phenolic hydroxyl groups and a lower content of carboxylic acid groups resulted in stronger adsorption affinity for corn stover lignin (CL) than for kenaf lignin (KL) and aspen lignin (AL). The lower amount of aliphatic hydroxyls that reduced hydrophobic interactions could explain the higher adsorption capacity of pine lignin (PL) than CL. Enzyme activity assays, as well as the hydrolysis of Avicel, phosphoric acid-swollen cellulose (PASC), and holocellulose, were performed to study the behaviors of mono-component enzymes that resulted in adsorption. We found that cellobiohydrolase (CBH) and xylanase were adsorbed the most by all lignins, endoglucanase (EG) showed less inhibition, and β-glucosidase (BG) was the least affected by lignins, indicating the important role of carbohydrate-binding module (CBM) in protein adsorption.
conclusionLignin sources affect enzyme adsorption using structural features and lignin composition, such as S/G ratio, carboxylic acid, aliphatic hydroxyl, and phenolic hydroxyl. For mono-component enzymes, the adsorption capacity decreased in the order CBH, xylanase > EG > BG. These investigations revealed the difference in lignin properties between diverse biomass and adsorption capacity of enzymes to lignins, and the possible underlying mechanism. The results can also serve as a reference for the genetic modification of lignocellulosic biomass and enzymes.
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