ArticleAnnals of laboratory medicine2014
Development of enzyme-linked immunosorbent assays using 2 truncated ORF2 proteins for detection of IgG antibodies against hepatitis E virus.
Article in Annals of laboratory medicine, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Who cites it
12 citing papers in PubMed, 23 citations in OpenAlex.
- Genetic Evolution of Hepatitis E Virus.Advances in experimental medicine and biology · 2023Review
- Advances in Hepatitis E Virus Biology and Pathogenesis.Viruses · 2021Review
- Development of new hepatitis E vaccines.Human vaccines & immunotherapeutics · 2018Review
- Article
- Detection of Serum Antibodies to Hepatitis E Virus Based on HEV Genotype 3 ORF2 Capsid Protein Expressed in Nicotiana benthamiana.Annals of laboratory medicine · 2017Article
- Epidemiology of Hepatitis E in Pregnant Women and Children in Iran: A General Overview.Journal of clinical and translational hepatology · 2016Review
- Epidemiology of hepatitis E virus in Iran.World journal of gastroenterology · 2016Review
- Design, Construction and Cloning of Truncated ORF2 and tPAsp-PADRE-Truncated ORF2 Gene Cassette From Hepatitis E Virus in the pVAX1 Expression Vector.Jundishapur journal of microbiology · 2015Article
- Hepatitis E Virus Seroprevalence Among Blood Donors in Bushehr, South of Iran.Hepatitis monthly · 2015Article
- Design and production of a multiepitope construct derived from hepatitis E virus capsid protein.Journal of medical virology · 2015Article
- Cytokine Profiles and Cell Proliferation Responses to Truncated ORF2 Protein in Iranian Patients Recovered from Hepatitis E Infection.Journal of tropical medicine · 2015Article
- Prevalence of Hepatitis E Virus among Adults in South-West of Iran.Hepatitis research and treatment · 2015Article
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundWithout appropriate culture systems for hepatitis E virus (HEV), sufficient natural viral proteins are difficult to generate for use in serological tests. Therefore, it is important to produce large amounts of HEV recombinant proteins in an economical way. The present study developed ELISAs using 2 truncated forms of the HEV open reading frame (ORF) 2 protein in order to detect anti-HEV IgG in serum samples.
methodsTwo truncated forms of the ORF2 protein were expressed in Escherichia coli and were purified by Ni(2+)-chelate-affinity chromatography (Qiagen, Germany). Two ELISAs were developed using these proteins and were compared with DIA.PRO HEV IgG ELISA kit (DIA.PRO. Italy) in 220 serum samples.
resultsHigh yields of the target proteins were obtained through codon optimization. The concentration and purity of the proteins were improved with Amicon filters (EMD Millipore, USA). Sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and Western blotting analysis of the resultant proteins showed a protein band of approximately 60 kDa corresponding to ORF2.1 (amino acids 112-660) and a protein band of approximately 55 kDa corresponding to ORF2.2 (amino acids 112-607). Positive agreement, negative agreement, and concordance of the 2 in-house ELISAs compared with DIA.PRO HEV IgG ELISA kit were 87%, 99.5%, and 98.1%, respectively (kappa=0.899, P=0.625).
conclusionsThe newly developed ELISAs are useful for detecting anti-HEV IgG in serum samples and are highly concordant with DIA.PRO HEV IgG ELISA kit.
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