Evidence map›Paper›PMID 24624312›Full record

ArticleInternational journal of burns and trauma2014

Isotopic study of L-Arginine kinetics in the lung during pseudomonas sepsis in an ovine model.

Hongzhi Xu, Davin Watson, Yong-Ming Yu, Daniel L Traber, Stefani Fischer, Joan Nichols, Donald Deyo, Lillian L Traber, Joaquin Cortiella

RetractedAbstract readRetracted Publication
PubMed Full text at PMC
In one paragraph

Article in International journal of burns and trauma, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. It has been retracted, and should not be counted. Not yet cited in PubMed.

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0citing papers in PubMed
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1 · What the graph read from it

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3 · Its place in the literature

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4 · The record

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5 · Who and what money

Authors and funding

9 authors.

Hongzhi XuShriners Hospital for Children and Massachusetts General Hospital Boston 02114, USA.
Davin WatsonUniversity of MassachusettsMedical School Worcester, MA 01655.
Yong-Ming YuShriners Hospital for Children and Massachusetts General Hospital Boston 02114, USA.
Daniel L TraberDepartment of Anesthesiology, The University of Texas Medical Branch and Shriners Hospital for Children Galveston, TX 77555, USA.
Stefani FischerDepartment of Anesthesiology, The University of Texas Medical Branch and Shriners Hospital for Children Galveston, TX 77555, USA.
Joan NicholsDepartment of Anesthesiology, The University of Texas Medical Branch and Shriners Hospital for Children Galveston, TX 77555, USA.
Donald DeyoDepartment of Anesthesiology, The University of Texas Medical Branch and Shriners Hospital for Children Galveston, TX 77555, USA.
Lillian L TraberDepartment of Anesthesiology, The University of Texas Medical Branch and Shriners Hospital for Children Galveston, TX 77555, USA.
Joaquin CortiellaDepartment of Anesthesiology, The University of Texas Medical Branch and Shriners Hospital for Children Galveston, TX 77555, USA.

Funding

ROLE OF DIETARY CONSTITUENTS ON GENE EXPRESSION IN INTESTINAL EPITHELIUMP30DK040561 · NIDDK · MASSACHUSETTS GENERAL HOSPITAL · PI CHRISTOPHER P DUGGAN · 1994 to 2026
$31.6M
NIDDK NIH HHS P30 DK040561
6 · The paper itself

Abstract

unlabelledThe objective of the study is to investigate how L-Arginine pulmonary metabolism is altered in response Pseudomonas aeruginosa (P. aeruginosa) induced septic conditions using an ovine model.

methodsSeven female sheep were infused with a primed-constant infusion of L-[(15)N2-guanidino, 5, 5, (2)H2] L-Arginine for 28 hs. After the initial 4 hs of the L-Arginine infusion, a continuous infusion of live Pseudomonas aeruginosa bacteria started for 24 hs. A NO synthase (NOS) inhibitor, N(G)-Methyl-L-arginine (L-NMA), infusion was added during the last 4 hs of the bacterial infusion. Blood samples were taken at specific time points for isotopic enrichment during control, septic and NOS blocking phases of the study.

resultsWe observed that the level of total delivery of L-Arginine to the lungs was significantly decreased in septic phase after 24 hours of pseudomonas infusion. In contrast, the fractional uptake and metabolism of L-Arginine by the lungs was doubled during septic phase relative to the control phase (MARG-basal = 100% vs. MARG-septic = 220 ± 56%, P < 0.05). NO production in the lungs was also significantly increased. Infusion of L-NMA markedly blunted this elevated NO production and attenuated the total arginine metabolized in the septic lungs (MARG-septic = 220 ± 56% vs. MARG-NO blocking = -25 ± 20%; P < 0.05). We demonstrated sepsis induced by P. aeruginosa infusion caused an increase in the fractional uptake and metabolic rate of arginine in the lungs. Furthermore, our data suggests that arginine was mainly consumed via arginine - NO pathway, which might be responsible for this enhanced arginine metabolic activity in the septic lungs.

Indexed as

Argininefractional uptakeisotopic studynitric oxidenitric oxide synthasePseudomonas aeruginosasepsis

Identifiers

PMID24624312
PMCPMC3945826

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.