ArticleMolecular vision2014
Adrenomedullin22-52 suppresses high-glucose-induced migration, proliferation, and tube formation of human retinal endothelial cells.
Article in Molecular vision, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed, 33 citations in OpenAlex.
- Adrenomedullin-RAMP2 System Modulates Inflammation and Tissue Repair in Experimental Autoimmune Uveitis Via T-Cell and M2 Macrophage Regulation.Investigative ophthalmology & visual science · 2025Article
- Mir-338-3p targeting THBS1 attenuates glioma progression by inhibiting the PI3K/Akt pathway.Biology direct · 2024Article
- Inhibition of viability of human retinal microvascular endothelial cells by vialinin A under high glucose condition.International journal of ophthalmology · 2024Article
- ECFC-derived exosomal THBS1 mediates angiogenesis and osteogenesis in distraction osteogenesis via the PI3K/AKT/ERK pathway.Journal of orthopaedic translation · 2022Article
- C-terminal binding protein 2 promotes high-glucose-triggered cell proliferation, angiogenesis and cellular adhesion of human retinal endothelial cell line.International ophthalmology · 2022Article
- SDF‑1α/CXCR4 signaling promotes capillary tube formation of human retinal vascular endothelial cells by activating ERK1/2 and PI3K pathwaysMolecular medicine reports · 2022Article
- Perivascular adipose tissue in autoimmune rheumatic diseases.Pharmacological research · 2022Review
- Long non-coding ribonucleic acid urothelial carcinoma-associated 1 promotes high glucose-induced human retinal endothelial cells angiogenesis through regulating micro-ribonucleic acid-624-3p/vascular endothelial growth factor C.Journal of diabetes investigation · 2021Article
- Assessing the protective effects of cryptotanshinone on CoClMolecular medicine reports · 2021Article
- Celastrol inhibits the proliferation and angiogenesis of high glucose-induced human retinal endothelial cells.Biomedical engineering online · 2021Article
- Silencing of miR-20b-5p Exerts Inhibitory Effect on Diabetic Retinopathy via Inactivation ofDiabetes, metabolic syndrome and obesity : targets and therapy · 2021Article
- Schizandrin A Protects Human Retinal Pigment Epithelial Cell Line ARPE-19 against HG-Induced Cell Injury by Regulation of miR-145.Molecular therapy. Nucleic acids · 2020Article
- Article
- Blockade of insulin receptor substrate-1 inhibits biological behavior of choroidal endothelial cells.International journal of ophthalmology · 2019Article
- Kaempferol targets estrogen-related receptor α and suppresses the angiogenesis of human retinal endothelial cells under high glucose conditions.Experimental and therapeutic medicine · 2017Article
- Hypoxia-Inducible Factor-1α Target Genes Contribute to Retinal Neuroprotection.Frontiers in cellular neuroscience · 2017Review
- Erianin inhibits high glucose-induced retinal angiogenesis via blocking ERK1/2-regulated HIF-1α-VEGF/VEGFR2 signaling pathway.Scientific reports · 2016Article
- High glucose concentration induces endothelial cell proliferation by regulating cyclin-D2-related miR-98.Journal of cellular and molecular medicine · 2016Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
purposeTo investigate the roles of an adrenomedullin receptor antagonist (adrenomedullin(22-52)) on high-glucose-induced human retinal endothelial cell (HREC) in vitro cell biology.
methodsHRECs were cultured with different concentrations of glucose and adrenomedullin(22-52). The proliferation of HRECs was evaluated by a cell counting kit-8 assay. Cell migration was assessed by scratch wound assay, and cell sprouting was detected by tube formation assay. The mRNA levels of adrenomedullin (ADM), vascular endothelial growth factor (VEGF), ADAMTS-1, and TSP-1 were measured by reverse-transcription polymerase chain reaction (RT-PCR). The VEGF and phosphatidylinositol 3' kinase (PI3K) pathway protein expression levels were assessed by western blot analysis.
resultsCompared with 5 mM normal glucose treatment, 30 mM glucose significantly promoted the migration of HRECs, which was attenuated by 1 μg/ml adrenomedullin(22-52). The proliferation of HRECs was also suppressed by 1 μg/ml adrenomedullin(22-52). Furthermore, compared with other groups, 5 μg/ml of adrenomedullin(22-52) was shown to suppress high-glucose-induced tube formation of HRECs. With adrenomedullin(22-52) treatment, the mRNA level of ADAMTS-1 was significantly increased. Moreover, western blot and RT-PCR analyses showed that HRECs treated with 30 mM glucose exhibited increased VEGF and PI3K pathway protein levels, while the expression levels were suppressed by 5 μg/ml of adrenomedullin(22-52).
conclusionsOur study indicated that adrenomedullin(22-52) mediated the migration, proliferation and tube formation after HRECs were exposed to high levels of glucose, which may be related to its ability to affect the expression of VEGF through the PI3K pathway.
Indexed as
Identifiers
24623968PMC3945807W2147735384What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.