ArticlePlant physiology2014
High-efficiency stable transformation of the model fern species Ceratopteris richardii via microparticle bombardment.
Article in Plant physiology, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 34 papers.
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Who cites it
34 citing papers in PubMed, 80 citations in OpenAlex.
- LEAFY demonstrates functions in reproductive development of the gametophyte but not the sporophyte of the fern Ceratopteris richardii.Development (Cambridge, England) · 2026Article
- Sex-type specification and meristem development in fern gametophytes.Discover developmental biology · 2026Review
- Non-seed plant research in the spotlight.Biology open · 2025Article
- Sexual reproduction in land plants: an evolutionary perspective.Plant reproduction · 2025Review
- Functions and Regulation of HAM Family Genes in Meristems During Gametophyte and Sporophyte Generations.Plant, cell & environment · 2025Review
- The cellular basis of meristem development in fern gametophytes.Biochemical Society transactions · 2025Review
- Single cell-derived multicellular meristem: insights into male-to-hermaphrodite conversion and de novo meristem formation in Ceratopteris.Development (Cambridge, England) · 2025Article
- Efficient gene editing of a model fern species through gametophyte-based transformation.Plant physiology · 2024Article
- Analysis of auxin responses in the fern Ceratopteris richardii identifies the developmental phase as a major determinant for response properties.Development (Cambridge, England) · 2024Article
- The role of fossils for reconstructing the evolution of plant development.Development (Cambridge, England) · 2024Article
- A conserved GRAS-domain transcriptional regulator links meristem indeterminacy to sex determination in Ceratopteris gametophytes.Current biology : CB · 2024Article
- Differential expression and co-localization of transcriptional factors during callus transition to differentiation for shoot organogenesis in the water fern Ceratopteris richardii.Annals of botany · 2024Article
- Polarized distribution of extracellular nucleotides promotes gravity-directed polarization of development in spores ofFrontiers in plant science · 2023Article
- What can hornworts teach us?Frontiers in plant science · 2023Review
- Positional cues and cell division dynamics drive meristem development and archegonium formation in Ceratopteris gametophytes.Communications biology · 2022Article
- The biology ofeLife · 2022Article
- Transcriptional analysis ofFrontiers in plant science · 2022Article
- An Agrobacterium-mediated stable transformation technique for the hornwort model Anthoceros agrestis.The New phytologist · 2021Article
- Charting the genomic landscape of seed-free plants.Nature plants · 2021Review
- Review
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Ferns represent the most closely related extant lineage to seed plants. The aquatic fern Ceratopteris richardii has been subject to research for a considerable period of time, but analyses of the genetic programs underpinning developmental processes have been hampered by a large genome size, a lack of available mutants, and an inability to create stable transgenic lines. In this paper, we report a protocol for efficient stable genetic transformation of C. richardii and a closely related species Ceratopteris thalictroides using microparticle bombardment. Indeterminate callus was generated and maintained from the sporophytes of both species using cytokinin treatment. In proof-of-principle experiments, a 35S::β-glucuronidase (GUS) expression cassette was introduced into callus cells via tungsten microparticles, and stable transformants were selected via a linked hygromycin B resistance marker. The presence of the transgene in regenerated plants and in subsequent generations was validated using DNA-blot analysis, reverse transcription-polymerase chain reaction, and GUS staining. GUS staining patterns in most vegetative tissues corresponded with constitutive gene expression. The protocol described in this paper yields transformation efficiencies far greater than those previously published and represents a significant step toward the establishment of a tractable fern genetic model.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.