ArticleThe Journal of neuroscience : the official journal of the Society for Neuroscience2014
Maturation of AMPAR composition and the GABAAR reversal potential in hPSC-derived cortical neurons.
Article in The Journal of neuroscience : the official journal of the Society for Neuroscience, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
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Who cites it
25 citing papers in PubMed.
- Transcriptomic and electrophysiological alterations underlying phenotypic variability in SCN1A-associated febrile seizures.Scientific reports · 2025Article
- Heterogeneous subpopulations of GABAiScience · 2024Article
- Neuropharmacology of human TERA2.cl.SP12 stem cell-derived neurons in ultra-long-term culture for antiseizure drug discovery.Frontiers in neuroscience · 2023Article
- An interaction between synapsin and C9orf72 regulates excitatory synapses and is impaired in ALS/FTD.Acta neuropathologica · 2022Article
- Human stem cell models of neurodegeneration: From basic science of amyotrophic lateral sclerosis to clinical translation.Cell stem cell · 2022Review
- Altered network properties in C9ORF72 repeat expansion cortical neurons are due to synaptic dysfunction.Molecular neurodegeneration · 2021Article
- Transactive response DNA-binding protein-43 proteinopathy in oligodendrocytes revealed using an induced pluripotent stem cell model.Brain communications · 2021Article
- Cortical neurons derived from human pluripotent stem cells lacking FMRP display altered spontaneous firing patterns.Molecular autism · 2020Article
- Article
- Neuronal Activity and Its Role in Controlling Antioxidant Genes.International journal of molecular sciences · 2020Review
- C9ORF72 repeat expansion causes vulnerability of motor neurons to CaNature communications · 2018Article
- Neurons and neuronal activity control gene expression in astrocytes to regulate their development and metabolism.Nature communications · 2017Article
- Functional properties of in vitro excitatory cortical neurons derived from human pluripotent stem cells.The Journal of physiology · 2016Review
- Improving and accelerating the differentiation and functional maturation of human stem cell-derived neurons: role of extracellular calcium and GABA.The Journal of physiology · 2016Review
- Are stem cell-derived neural cells physiologically credible?The Journal of physiology · 2016Article
- Effects of Ethanol on Cellular Composition and Network Excitability of Human Pluripotent Stem Cell-Derived Neurons.Alcoholism, clinical and experimental research · 2016Article
- Evidence for evolutionary divergence of activity-dependent gene expression in developing neurons.eLife · 2016Article
- Gain of BDNF Function in Engrafted Neural Stem Cells Promotes the Therapeutic Potential for Alzheimer's Disease.Scientific reports · 2016Article
- Maturation and electrophysiological properties of human pluripotent stem cell-derived oligodendrocytes.Stem cells (Dayton, Ohio) · 2016Article
- Functional Properties of Human Stem Cell-Derived Neurons in Health and Disease.Stem cells international · 2016Review
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9 authors.
Funding
Abstract
Rodent-based studies have shown that neurons undergo major developmental changes to ion channel expression and ionic gradients that determine their excitation-inhibition balance. Neurons derived from human pluripotent stem cells theoretically offer the potential to study classical developmental processes in a human-relevant system, although this is currently not well explored. Here, we show that excitatory cortical-patterned neurons derived from multiple human pluripotent stem cell lines exhibit native-like maturation changes in AMPAR composition such that there is an increase in the expression of GluA2(R) subunits. Moreover, we observe a dynamic shift in intracellular Cl- levels, which determines the reversal potential of GABAAR-mediated currents and is influenced by neurotrophic factors. The shift is concomitant with changes in KCC2 and NKCC1 expression. Because some human diseases are thought to involve perturbations to AMPAR GluA2 content and others in the chloride reversal potential, human stem-cell-derived neurons represent a valuable tool for studying these fundamental properties.
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