ArticleProtein science : a publication of the Protein Society2014
A synthetic biochemistry system for the in vitro production of isoprene from glycolysis intermediates.
Article in Protein science : a publication of the Protein Society, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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20 citing papers in PubMed, 66 citations in OpenAlex.
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- Exploring Emergent Properties in Enzymatic Reaction Networks: Design and Control of Dynamic Functional Systems.Chemical reviews · 2024Review
- A pilot study for the prediction of liver function related scores using breath biomarkers and machine learning.Scientific reports · 2022Article
- Key reaction components affect the kinetics and performance robustness of cell-free protein synthesis reactions.Computational and structural biotechnology journal · 2022Article
- Transmetabolism: the non-conformist approach to biotechnology.Microbial biotechnology · 2021Article
- A cell-free system for production of 2,3-butanediol is robust to growth-toxic compounds.Metabolic engineering communications · 2020Article
- Cell-Free Synthetic Glycobiology: Designing and Engineering Glycomolecules Outside of Living Cells.Frontiers in chemistry · 2020Review
- Enzyme alchemy: cell-free synthetic biochemistry for natural products.Emerging topics in life sciences · 2019Article
- Synthetic Biology Goes Cell-Free.BMC biology · 2019Review
- Cell-free biosynthesis of limonene using enzyme-enrichedSynthetic biology (Oxford, England) · 2019Article
- Conversion of d-glucose to l-lactate via pyruvate by an optimized cell-free enzymatic biosystem containing minimized reactions.Synthetic and systems biotechnology · 2018Article
- A molecular rheostat maintains ATP levels to drive a synthetic biochemistry system.Nature chemical biology · 2017Article
- Modules forSynthetic and systems biotechnology · 2017Review
- A synthetic biochemistry platform for cell free production of monoterpenes from glucose.Nature communications · 2017Article
- Biomanufacturing: history and perspective.Journal of industrial microbiology & biotechnology · 2017Review
- Enzymatic process optimization for the in vitro production of isoprene from mevalonate.Microbial cell factories · 2017Article
- Cell-Free Mixing of Escherichia coli Crude Extracts to Prototype and Rationally Engineer High-Titer Mevalonate Synthesis.ACS synthetic biology · 2016Article
- Forward design of a complex enzyme cascade reaction.Nature communications · 2016Article
- Cell-free metabolic engineering: biomanufacturing beyond the cell.Biotechnology journal · 2015Review
- Evidence of a novel mevalonate pathway in archaea.Biochemistry · 2014Article
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Authors and funding
6 authors at 2 institutions in 1 country.
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No grant is acknowledged in the PubMed record.
Abstract
The high yields required for the economical production of chemicals and fuels using microbes can be difficult to achieve due to the complexities of cellular metabolism. An alternative to performing biochemical transformations in microbes is to build biochemical pathways in vitro, an approach we call synthetic biochemistry. Here we test whether the full mevalonate pathway can be reconstituted in vitro and used to produce the commodity chemical isoprene. We construct an in vitro synthetic biochemical pathway that uses the carbon and ATP produced from the glycolysis intermediate phosphoenolpyruvate to run the mevalonate pathway. The system involves 12 enzymes to perform the complex transformation, while providing and balancing the ATP, NADPH, and acetyl-CoA cofactors. The optimized system produces isoprene from phosphoenolpyruvate in ∼100% molar yield. Thus, by inserting the isoprene pathway into previously developed glycolysis modules it may be possible to produce isoprene and other acetyl-CoA derived isoprenoids from glucose in vitro.
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