ArticleJournal of virology2014
The NSs protein of tomato spotted wilt virus is required for persistent infection and transmission by Frankliniella occidentalis.
Article in Journal of virology, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 34 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
34 citing papers in PubMed, 95 citations in OpenAlex.
- Molecular characterization and comparison of tomato zonate spot virus isolated in Japan and China.Virus genes · 2024Article
- Article
- Tagitinin A regulates an F-box gene, CPR30, to resist tomato spotted wilt orthotospovirus (TSWV) infection in Nicotiana benthamiana.PloS one · 2024Article
- Signaling and Resistosome Formation in Plant Innate Immunity to Viruses: Is There a Common Mechanism of Antiviral Resistance Conserved across Kingdoms?International journal of molecular sciences · 2023Review
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- Article
- The nonstructural protein NSs encoded by tomato zonate spot virus suppresses RNA silencing by interacting with NbSGS3.Molecular plant pathology · 2022Article
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- The Infection Route of Tomato Zonate Spot Virus in the Digestive System of Its Insect VectorFrontiers in microbiology · 2022Article
- Article
- Complexity and Local Specificity of the Virome Associated with Tospovirus-Transmitting Thrips Species.Journal of virology · 2021Article
- Antiviral RISC mainly targets viral mRNA but not genomic RNA of tospovirus.PLoS pathogens · 2021Article
- The Bunyavirales: The Plant-Infecting Counterparts.Viruses · 2021Review
- Current Status and Potential of RNA Interference for the Management of Tomato Spotted Wilt Virus and Thrips Vectors.Pathogens (Basel, Switzerland) · 2021Review
- Applications of Proteomic Tools to Study Insect Vector-Plant Virus Interactions.Life (Basel, Switzerland) · 2020Review
- Relationships of virus titers and transmission rates among sympatric and allopatric virus isolates and thrips vectors support local adaptation.Scientific reports · 2020Article
- Rescue of tomato spotted wilt virus entirely from complementary DNA clones.Proceedings of the National Academy of Sciences of the United States of America · 2020Article
- NSs, the Silencing Suppressor of Tomato Spotted Wilt Orthotospovirus, Interferes With JA-Regulated Host Terpenoids Expression to AttractFrontiers in microbiology · 2020Article
- Marine Oomycetes of the GenusFrontiers in microbiology · 2020Article
- The Orthotospovirus nonstructural protein NSs suppresses plant MYC-regulated jasmonate signaling leading to enhanced vector attraction and performance.PLoS pathogens · 2019Article
Corrections and comments
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Authors and funding
7 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
unlabelledTomato spotted wilt virus (TSWV) is the type member of tospoviruses (genus Tospovirus), plant-infecting viruses that cause severe damage to ornamental and vegetable crops. Tospoviruses are transmitted by thrips in the circulative propagative mode. We generated a collection of NSs-defective TSWV isolates and showed that TSWV coding for truncated NSs protein could not be transmitted by Frankliniella occidentalis. Quantitative reverse transcription (RT)-PCR and immunostaining of individual insects detected the mutant virus in second-instar larvae and adult insects, demonstrating that insects could acquire and accumulate the NSs-defective virus. Nevertheless, adults carried a significantly lower viral load, resulting in the absence of transmission. Genome sequencing and analyses of reassortant isolates showed genetic evidence of the association between the loss of competence in transmission and the mutation in the NSs coding sequence. Our findings offer new insight into the TSWV-thrips interaction and Tospovirus pathogenesis and highlight, for the first time in the Bunyaviridae family, a major role for the S segment, and specifically for the NSs protein, in virulence and efficient infection in insect vector individuals. IMPORTANCE: Our work is the first to show a role for the NSs protein in virus accumulation in the insect vector in the Bunyaviridae family: demonstration was obtained for the system TSWV-F. occidentalis, arguably one of the most damaging combination for vegetable crops. Genetic evidence of the involvement of the NSs protein in vector transmission was provided with multiple approaches.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.