ArticleJournal of clinical microbiology2014
Application of qualitative and quantitative real-time PCR, direct sequencing, and terminal restriction fragment length polymorphism analysis for detection and identification of polymicrobial 16S rRNA genes in ascites.
Article in Journal of clinical microbiology, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
9 citing papers in PubMed.
- Molecular quantification and differentiation of Candida species in biological specimens of patients with liver cirrhosis.PloS one · 2018Trial
- Changes of the bacterial composition in duodenal fluid from patients with liver cirrhosis and molecular bacterascites.Scientific reports · 2023Article
- Comparison of an automated DNA extraction and 16S rDNA real time PCR/sequencing diagnostic method using optimized reagents with culture during a 15-month study using specimens from sterile body sites.BMC microbiology · 2022Article
- Automated 16S Sequencing Using an R-Based Analysis Module for Bacterial Identification.Microbiology spectrum · 2022Article
- Amplification of bacterial genomic DNA from all ascitic fluids with a highly sensitive polymerase chain reaction.Molecular medicine reports · 2018Article
- Management of Infectious Complications Associated with Acute-on-Chronic Liver Failure.Visceral medicine · 2018Review
- Bacterial translocation markers in liver cirrhosis.Annals of gastroenterology · 2017Review
- Use of Bacterial DNA Concentration in Ascites as a Marker for Spontaneous Bacterial Peritonitis.Journal of clinical and experimental hepatologyArticle
- Changing the Dial on Pathogen Detection in SBP.Journal of clinical and experimental hepatologyArticle
Corrections and comments
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Authors and funding
10 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Qualitative and quantitative 16S rRNA gene-based real-time PCR and direct sequencing were applied for rapid detection and identification of bacterial DNA (bactDNA) in 356 ascites samples. bactDNA was detected in 35% of samples, with a mean of 3.24 log copies ml(-1). Direct sequencing of PCR products revealed 62% mixed chromatograms predominantly belonging to Gram-positive bacteria. Terminal restriction fragment length polymorphism (T-RFLP) results of a sample subset confirmed sequence data showing polymicrobial DNA contents in 67% of bactDNA-positive ascites samples.
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