ArticleJournal of clinical microbiology2014
Multilocus sequence typing of Mycoplasma hyorhinis strains identified by a real-time TaqMan PCR assay.
Article in Journal of clinical microbiology, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
24 citing papers in PubMed.
- Mycoplasma infection drives EGFR-TKI resistance through convergent transcriptomic reprogramming in non-small cell lung cancer.iScience · 2026Article
- Epidemiological study of risk factors from nursery and growing-finishing pig farms associated with rate ratio of pleuritis at slaughterhouse in the state of Santa Catarina, Brazil.Veterinary research communications · 2026Article
- Biological characterization, sequence type distribution and drug resistance profiling ofFrontiers in veterinary science · 2026Article
- Exploring the pangenome of Mycoplasma hyorhinis in search of potential virulence markers.Scientific reports · 2025Article
- Molecular Survey of Selected Bacterial Respiratory Pathogens in Polish Wild Boars.Pathogens (Basel, Switzerland) · 2025Article
- Detection of infectious agents in lungs of slaughtered pigs in association with cranioventral pulmonary consolidation.Journal of veterinary research · 2025Article
- EAVLD 2024 - 7Italian journal of food safety · 2024Article
- Development of a new multiplex quantitative PCR for the detection of Glaesserella parasuis, Mycoplasma hyorhinis, and Mycoplasma hyosynoviae.MicrobiologyOpen · 2023Article
- Article
- Bacterial polyarthritis in post-weaning pigs in a high-density swine breeding area in Italy.Journal of veterinary diagnostic investigation : official publication of the American Association of Veterinary Laboratory Diagnosticians, Inc · 2022Article
- The role of co-infections in M. hyopneumoniae outbreaks among heavy fattening pigs: a field study.Veterinary research · 2022Article
- Evaluating the presence of Mycoplasma hyorhinis, Fusobacterium nucleatum, and Helicobacter pylori in biopsies of patients with gastric cancer.Infectious agents and cancer · 2021Article
- Multilocus sequence typing of pathogenic Mycoplasma mycoides subsp. capri reveals the predominance of a novel clonal complex among isolates from goats in India.Archives of microbiology · 2021Article
- Swine Conjunctivitis Associated with a Novel Mycoplasma Species Closely Related toPathogens (Basel, Switzerland) · 2020Article
- Causes of swine polyserositis in a high-density breeding area in Italy.Journal of veterinary diagnostic investigation : official publication of the American Association of Veterinary Laboratory Diagnosticians, Inc · 2020Article
- Multilocus sequence typing characterizes diversity of Ureaplasma diversum strains, and intra-species variability induces different immune response profiles.BMC veterinary research · 2020Article
- Development of a one-run real-time PCR detection system for pathogens associated with porcine respiratory diseases.The Journal of veterinary medical science · 2020Article
- Association Between Infectious Agents and Lesions in Post-Weaned Piglets and Fattening Heavy Pigs With Porcine Respiratory Disease Complex (PRDC).Frontiers in veterinary science · 2020Article
- Genetic structure of Mycoplasma ovipneumoniae informs pathogen spillover dynamics between domestic and wild Caprinae in the western United States.Scientific reports · 2019Article
- Molecular epidemiology of Mycoplasma hyorhinis porcine field isolates in the United States.PloS one · 2019Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors.
Funding
Abstract
A real-time TaqMan PCR assay based on the gene encoding the protein p37 was developed to detect Mycoplasma hyorhinis. Its specificity was validated with 29 epidemiologically unrelated M. hyorhinis strains (28 field strains and one reference strain) and other mycoplasma species or with other microorganisms commonly found in pigs. The estimated detection limit of this qPCR assay was 125 microorganism equivalents/μl. The same 29 epidemiologically unrelated M. hyorhinis strains and four previously fully sequenced strains were typed by two portable typing methods, the sequencing of the p37 gene and a multilocus sequence typing (MLST) scheme. The first method revealed 18 distinct nucleotide sequences and insufficient discriminatory power (0.934). The MLST scheme was developed with the sequenced genomes of the M. hyorhinis strains HUB-1, GDL-1, MCLD, and SK76 and based on the genes dnaA, rpoB, gyrB, gltX, adk, and gmk. In total, 2,304 bp of sequence was analyzed for each strain. MLST was capable of subdividing the 33 strains into 29 distinct sequence types. The discriminatory power of the method was >0.95, which is the threshold value for interpreting typing results with confidence (D=0.989). Population analysis showed that recombination in M. hyorhinis occurs and that strains are diverse but with a certain clonality (one unique clonal complex was identified). The new qPCR assay and the robust MLST scheme are available for the acquisition of new knowledge on M. hyorhinis epidemiology. A web-accessible database has been set up for the M. hyorhinis MLST scheme at http://pubmlst.org/mhyorhinis/.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.