ArticleBiology of reproduction2014
In vitro fertilization affects growth and glucose metabolism in a sex-specific manner in an outbred mouse model.
Article in Biology of reproduction, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 65 papers, 3 of them syntheses that pooled it.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
65 citing papers in PubMed, 3 syntheses or guidelines pooled it, 127 citations in OpenAlex.
- A Systematic Review on Biomarkers for Gestational Diabetes Mellitus Detection in Pregnancies Conceived Using Assisted Reproductive Technology: Current Trends and Future Directions.International journal of molecular sciences · 2025Pooled it
- Infertility treatment and offspring blood pressure-a systematic review and meta-analysis.Human reproduction update · 2025Pooled it
- Offspring physiology following the use of IVM, IVF and ICSI: a systematic review and meta-analysis of animal studies.Human reproduction update · 2023Pooled it
- Comparison of DNA methylation patterns of parentally imprinted genes in placenta derived from IVF conceptions in two different culture media.Human reproduction (Oxford, England) · 2020Trial
- Epigenetic, environmental, and sex-specific programming of long-term health in Assisted Reproductive Technologies-conceived offspring†.Biology of reproduction · 2026Review
- Ultra-low oxygen tension duringbioRxiv : the preprint server for biology · 2026Article
- Mouse placentae generated by in vitro fertilization exhibit altered gene expression, activated hypoxia responses, and reduced fitness†.Biology of reproduction · 2026Article
- When less really is more: reducing embryo manipulation to lower metabolic stress and improving long term outcomes.Reproduction, fertility, and development · 2026Review
- How Early-Life Programming During Embryogenesis Imprints Cellular Memory.International journal of molecular sciences · 2025Review
- IVF and Thermal Manipulation at the First Cleavage Stage Alter Offspring Circadian Phenotype, Sleep, and Brain Epigenetics.International journal of molecular sciences · 2025Article
- In Vitro Fertilization Accelerates Female Reproductive Aging Through Early Ovarian Failure.bioRxiv : the preprint server for biology · 2025Article
- Impact of embryo culture and day of embryo transfer on the cardiometabolic health of adult mice†.Biology of reproduction · 2025Article
- Melatonin Rescues Hyperacetylation of Liver and Impaired Enzymatic Activities of Mitochondrial in IVF Offspring.Reproductive sciences (Thousand Oaks, Calif.) · 2025Article
- Oxygen level alters energy metabolism in bovine preimplantation embryos.Scientific reports · 2025Article
- Proteomic and metabolomic insights into oxidative stress response activation in mouse embryos generated byHuman reproduction open · 2025Article
- Cryopreservation, cryoprotectants, and potential risk of epigenetic alteration.Journal of assisted reproduction and genetics · 2024Review
- Sex-specific effects ofJournal of molecular and cellular cardiology plus · 2023Article
- Long-Term Effects of ART on the Health of the Offspring.International journal of molecular sciences · 2023Review
- Comparative Proteomic and Phospho-proteomic Analysis of Mouse Placentas Generated via In Vivo and In Vitro Fertilization.Reproductive sciences (Thousand Oaks, Calif.) · 2023Article
- Effects of assisted reproductive technology on gene expression in heart and spleen tissues of adult offspring mouse.Frontiers in endocrinology · 2023Article
5 more citing papers are in PubMed but not listed here.
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
Abstract
The preimplantation period is a time of reprogramming that may be vulnerable to disruption. This question has wide clinical relevance since the number of children conceived by in vitro fertilization (IVF) is rising. To examine this question, outbred mice (CF1 × B6D2F1) conceived by IVF and cultured using Whitten medium and 20% O2 (IVFWM group, less optimal) or K simplex optimized medium with amino acids and 5% O2 (IVFKAA group, more optimal and similar to conditions used in human IVF) were studied postnatally. We found that flushed blastocysts transferred to recipient mice provided the best control group (FB group), as this accounted for the effects of superovulation, embryo transfer, and litter size. We observed that many physiological parameters were normal. Reassuringly, IVFKAA offspring did not differ significantly from FB offspring. However, male IVFWM mice (but not females) were larger during the first 19 wk of life and exhibited glucose intolerance. Male IVFWM mice also showed enlarged left heart despite normal blood pressure. Expression of candidate imprinted genes (H19, Igf2, and Slc38a4) in multiple adult tissues did not show differences among the groups; only Slc38a4 was down-regulated following IVF (in both culture conditions) in female adipose tissue. These studies demonstrate that adult metabolism is affected by the type of conditions encountered during the preimplantation stage. Further, the postnatal growth trajectory and glucose homeostasis following ex vivo manipulation may be sexual dimorphic. Future work on the long-term effects of IVF offspring should focus on glucose metabolism and the cardiovascular system.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.