ArticleJournal of experimental botany2014
Arabidopsis thaliana RALF1 opposes brassinosteroid effects on root cell elongation and lateral root formation.
Article in Journal of experimental botany, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 59 papers.
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Who cites it
59 citing papers in PubMed, 136 citations in OpenAlex.
- Plant Peptides on the Rise: From Historical Insight to Future Applications.Plant biotechnology journal · 2026Review
- The RALF-FERONIA Signaling Axis: A Central Hub Integrating Plant Growth, Reproduction, and Stress Responses.International journal of molecular sciences · 2025Review
- From roots to reproduction: the multifaceted roles of rapid alkalinization factor and epidermal patterning factor peptides in plants.Journal of experimental botany · 2025Review
- Transcriptome and Metabolomics Analysis Reveal the Effects of Red and Blue Light on the Physiology and Primary Medicinal Components (Liquiritin and Glycyrrhizic Acid) ofInternational journal of molecular sciences · 2025Article
- Jack of all trades: crosstalk between FERONIA signaling and hormone pathways.Journal of experimental botany · 2025Review
- A C-Terminally Encoded Peptide, MeCEP6, Promotes Nitrate Uptake in Cassava Roots.Plants (Basel, Switzerland) · 2025Article
- FERONIA signaling maintains cell wall integrity during brassinosteroid-induced cell expansion in Arabidopsis.Molecular plant · 2025Article
- Peptides and Reactive Oxygen Species Regulate Root Development.International journal of molecular sciences · 2025Review
- Peptide hormones in plants.Molecular horticulture · 2025Review
- RAPID ALKALINIZATION FACTOR 22 is a key modulator of the root hair growth responses to fungal ethylene emissions in Arabidopsis.Plant physiology · 2024Article
- FERONIA: A Receptor Kinase at the Core of a Global Signaling Network.Annual review of plant biology · 2024Review
- The RALF signaling pathway regulates cell wall integrity during pollen tube growth in maize.The Plant cell · 2024Article
- Growth-inhibiting effects of the unconventional plant APYRASE 7 of Arabidopsis thaliana influences the LRX/RALF/FER growth regulatory module.PLoS genetics · 2024Article
- Genetic regulation of lateral root development.Plant signaling & behavior · 2023Review
- Characterization of Malectin/Malectin-like Receptor-like Kinase Family Members in Foxtail Millet (Life (Basel, Switzerland) · 2023Article
- Rapid alkalinization factor: function, regulation, and potential applications in agriculture.Stress biology · 2023Review
- Lateral Root Initiation in Cucumber (International journal of molecular sciences · 2023Article
- Linking plant functional genes to rhizosphere microbes: a review.Plant biotechnology journal · 2023Review
- Integrative Proteomics and Metabolomics Analysis Reveals the Role of Small Signaling Peptide Rapid Alkalinization Factor 34 (RALF34) in Cucumber Roots.International journal of molecular sciences · 2023Article
- Molecular Mechanisms of Regulation of Root Development by Plant Peptides.Plants (Basel, Switzerland) · 2023Review
Corrections and comments
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Authors and funding
6 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Rapid alkalinization factor (RALF) is a peptide signal that plays a basic role in cell biology and most likely regulates cell expansion. In this study, transgenic Arabidopsis thaliana lines with high and low levels of AtRALF1 transcripts were used to investigate this peptide's mechanism of action. Overexpression of the root-specific isoform AtRALF1 resulted in reduced cell size. Conversely, AtRALF1 silencing increased root length by increasing the size of root cells. AtRALF1-silenced plants also showed an increase in the number of lateral roots, whereas AtRALF1 overexpression produced the opposite effect. In addition, four AtRALF1-inducible genes were identified: two genes encoding proline-rich proteins (AtPRP1 and AtPRP3), one encoding a hydroxyproline-rich glycoprotein (AtHRPG2), and one encoding a xyloglucan endotransglucosylase (TCH4). These genes were expressed in roots and involved in cell-wall rearrangement, and their induction was concentration dependent. Furthermore, AtRALF1-overexpressing plants were less sensitive to exogenous brassinolide (BL); upon BL treatment, the plants showed no increase in root length and a compromised increase in hypocotyl elongation. In addition, the treatment had no effect on the number of emerged lateral roots. AtRALF1 also induces two brassinosteroid (BR)-downregulated genes involved in the BR biosynthetic pathway: the cytochrome P450 monooxygenases CONSTITUTIVE PHOTOMORPHISM AND DWARFISM (CPD) and DWARF4 (DWF4). Simultaneous treatment with both AtRALF1 and BL caused a reduction in AtRALF1-inducible gene expression levels, suggesting that these signals may compete for components shared by both pathways. Taken together, these results indicate an opposing effect of AtRALF1 and BL, and suggest that RALF's mechanism of action could be to interfere with the BR signalling pathway.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.