ArticleThe Plant cell2014
Complete proteomic-based enzyme reaction and inhibition kinetics reveal how monolignol biosynthetic enzyme families affect metabolic flux and lignin in Populus trichocarpa.
Article in The Plant cell, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 74 papers.
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Who cites it
74 citing papers in PubMed.
- Turbo-charging crop improvement: harnessing multiplex editing for polygenic trait engineering and beyond.The Plant journal : for cell and molecular biology · 2025Review
- De novo biosynthesis of plant lignans by synthetic yeast consortia.Nature chemical biology · 2025Article
- The role of PagC3H3 in lignin biosynthesis and insect resistance in Populus alba × Populus glandulosa.Plant cell reports · 2025Article
- A Guide to Metabolic Network Modeling for Plant Biology.Plants (Basel, Switzerland) · 2025Review
- Epigenetic regulation of lignin biosynthesis in wood formation.The New phytologist · 2025Article
- Applications of CRISPR Technologies in Forestry and Molecular Wood Biotechnology.International journal of molecular sciences · 2024Review
- Synthetic-biology approach for plant lignocellulose engineering.Plant biotechnology (Tokyo, Japan) · 2024Article
- Expression of dehydroshikimate dehydratase in poplar induces transcriptional and metabolic changes in the phenylpropanoid pathway.Journal of experimental botany · 2024Article
- Multiplex CRISPR editing of wood for sustainable fiber production.Science (New York, N.Y.) · 2023Article
- Article
- Evidence for poplar PtaPLATZ18 in the regulation of plant growth and vascular tissues development.Frontiers in plant science · 2023Article
- Genome-wide identification and expression profiling of B3 transcription factor genes inFrontiers in plant science · 2023Article
- Lignin engineering in forest trees: From gene discovery to field trials.Plant communications · 2022Review
- Transcriptional Reactivation of Lignin Biosynthesis for the Heterologous Production of Etoposide Aglycone inACS synthetic biology · 2022Article
- Overexpression of the scopoletin biosynthetic pathway enhances lignocellulosic biomass processing.Science advances · 2022Article
- A new approach to zip-lignin: 3,4-dihydroxybenzoate is compatible with lignification.The New phytologist · 2022Article
- Engineering cofactor supply and recycling to drive phenolic acid biosynthesis in yeast.Nature chemical biology · 2022Article
- Dimerization of PtrMYB074 and PtrWRKY19 mediates transcriptional activation of PtrbHLH186 for secondary xylem development in Populus trichocarpa.The New phytologist · 2022Article
- Systematic Analysis and Biochemical Characterization of the Caffeoyl Shikimate Esterase Gene Family in Poplar.International journal of molecular sciences · 2021Article
- CRISPR-Cas9 editing of CAFFEOYL SHIKIMATE ESTERASE 1 and 2 shows their importance and partial redundancy in lignification in Populus tremula × P. alba.Plant biotechnology journal · 2021Article
14 more citing papers are in PubMed but not listed here.
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15 authors.
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Abstract
We established a predictive kinetic metabolic-flux model for the 21 enzymes and 24 metabolites of the monolignol biosynthetic pathway using Populus trichocarpa secondary differentiating xylem. To establish this model, a comprehensive study was performed to obtain the reaction and inhibition kinetic parameters of all 21 enzymes based on functional recombinant proteins. A total of 104 Michaelis-Menten kinetic parameters and 85 inhibition kinetic parameters were derived from these enzymes. Through mass spectrometry, we obtained the absolute quantities of all 21 pathway enzymes in the secondary differentiating xylem. This extensive experimental data set, generated from a single tissue specialized in wood formation, was used to construct the predictive kinetic metabolic-flux model to provide a comprehensive mathematical description of the monolignol biosynthetic pathway. The model was validated using experimental data from transgenic P. trichocarpa plants. The model predicts how pathway enzymes affect lignin content and composition, explains a long-standing paradox regarding the regulation of monolignol subunit ratios in lignin, and reveals novel mechanisms involved in the regulation of lignin biosynthesis. This model provides an explanation of the effects of genetic and transgenic perturbations of the monolignol biosynthetic pathway in flowering plants.
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