ArticleChinese journal of integrative medicine2014
Icariin promotes self-renewal of neural stem cells: an involvement of extracellular regulated kinase signaling pathway.
Article in Chinese journal of integrative medicine, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
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Who cites it
11 citing papers in PubMed, 23 citations in OpenAlex.
- Review
- Natural compounds from traditional Chinese medicine regulating stem cell fate: Identification and therapeutic potential.Chinese herbal medicines · 2025Review
- The potential of plant extracts in cell therapy.Stem cell research & therapy · 2022Review
- Deciphering the myth of icariin and synthetic derivatives in improving erectile function from a molecular biology perspective: a narrative review.Translational andrology and urology · 2022Review
- Bioactive Icariin/β-CD-IC/Bacterial Cellulose with Enhanced Biomedical Potential.Nanomaterials (Basel, Switzerland) · 2021Article
- Traditional Chinese Medicine Monomers: Novel Strategy for Endogenous Neural Stem Cells Activation After Stroke.Frontiers in cellular neuroscience · 2021Review
- Icariin and Its Metabolites as Potential Protective Phytochemicals Against Alzheimer's Disease.Frontiers in pharmacology · 2019Review
- The Effect of Traditional Chinese Medicine on Neural Stem Cell Proliferation and Differentiation.Aging and disease · 2017Review
- Icariin improves acute kidney injury and proteinuria in a rat model of pregnancy‑induced hypertension.Molecular medicine reports · 2017Article
- Icariin protects rats against 5/6 nephrectomy-induced chronic kidney failure by increasing the number of renal stem cells.BMC complementary and alternative medicine · 2015Article
- Icariin induces synoviolin expression through NFE2L1 to protect neurons from ER stress-induced apoptosis.PloS one · 2015Article
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
objectiveTo investigate the effects and underlying molecular mechanisms of icariin (ICA) on self-renewal and differentiation of neural stem cells (NSCs).
methodsNSCs were derived from forebrains of mice embryos by mechanical dissociation into single cell suspension. The self-renewal of NSCs was measured by neurosphere formation assay. The proliferation of NSCs was detected by water-soluble tetrazolium (WST) and 5-ethynyl-2'-deoxyuridine (EdU) incorporation assay. Protein expression of neuron-specific marker tubulin-βIII(TuJ1) and astrocyte-specific marker glial fibrillary acidic protein (GFAP) were measured by immunofluorescence and Western blotting. Using microarray, the differentially expressed genes (DEGs) were screened between NSCs with or without ICA treatment. The signaling pathways enriched by these DEGs and their role in mediating effects of ICA were analyzed.
resultsICA significantly promoted neurosphere formation of NSCs cultured in growth protocol in a dose-dependent manner and achieved the maximum effects at 100 nmol/L. ICA also increased optical absorbance value and EdU incorporation into nuclei of NSCs. ICA had no significant effects on the percentage of TuJ1 or GFAP-positive cells, and TuJ1 or GFAP protein expression in NSCs cultured in differentiation protocol. A total of 478 genes were found to be differentially regulated. Among signaling pathways significantly enriched by DEGs, mitogen activated protein kinase (MAPK) pathway was of interest. Blockade of extracellular signal-regulated kinase (ERK)/MAPK, other than p38/MAPK subfamily pathway partially abolished effects of ICA on neurosphere formation and EdU incorporation of NSCs.
conclusionICA can promote the selfrenewal of NSCs at least partially through ERK/MAPK signaling pathway.
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