ArticleInternational journal of molecular sciences2014
Detection of borreliae in archived sera from patients with clinically suspect Lyme disease.
Article in International journal of molecular sciences, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 33 citations in OpenAlex.
- Development of a Syndromic Molecular Diagnostic Assay for Tick-Borne Pathogens Using Barcoded Magnetic Bead Technology.Microbiology spectrum · 2023Article
- Quantitative multiplexed strategies for human Lyme disease serological testing.Experimental biology and medicine (Maywood, N.J.) · 2021Review
- Article
- A Practical Approach to the Diagnosis of Lyme Borreliosis: From Clinical Heterogeneity to Laboratory Methods.Frontiers in medicine · 2020Review
- Article
- Lyme Disease Frontiers: ReconcilingPathogens (Basel, Switzerland) · 2019Review
- Line Immunoblot Assay for Tick-Borne Relapsing Fever and Findings in Patient Sera from Australia, Ukraine and the USA.Healthcare (Basel, Switzerland) · 2019Article
- Single Core Genome Sequencing for Detection of bothInternational journal of environmental research and public health · 2019Article
- Direct Diagnostic Tests for Lyme Disease.Clinical infectious diseases : an official publication of the Infectious Diseases Society of America · 2019Review
- Precision medicine: retrospective chart review and data analysis of 200 patients on dapsone combination therapy for chronic Lyme disease/post-treatment Lyme disease syndrome: part 1.International journal of general medicine · 2019Article
- Host Immune Evasion by Lyme and Relapsing Fever Borreliae: Findings to Lead Future Studies forFrontiers in immunology · 2017Review
- Lyme disease caused by Borrelia burgdorferi with two homeologous 16S rRNA genes: a case report.International medical case reports journal · 2016Article
- Evaluation of two commercially available rapid diagnostic tests for Lyme borreliosis.European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology · 2015Article
- DNA sequencing diagnosis of off-season spirochetemia with low bacterial density in Borrelia burgdorferi and Borrelia miyamotoi infections.International journal of molecular sciences · 2014Article
- Relevance of chronic lyme disease to family medicine as a complex multidimensional chronic disease construct: a systematic review.International journal of family medicine · 2014Review
- Lyme and associated tick-borne diseases: global challenges in the context of a public health threat.Frontiers in cellular and infection microbiology · 2014Review
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The diagnoses of Lyme disease based on clinical manifestations, serological findings and detection of infectious agents often contradict each other. We tested 52 blind-coded serum samples, including 20 pre-treatment and 12 post-treatment sera from clinically suspect Lyme disease patients, for the presence of residual Lyme disease infectious agents, using nested PCR amplification of a signature segment of the borrelial 16S ribosomal RNA gene for detection and direct DNA sequencing of the PCR amplicon for molecular validation. These archived sera were split from the samples drawn for the 2-tier serology tests performed by a CDC-approved laboratory, and are used as reference materials for evaluating new diagnostic reagents. Of the 12 post-treatment serum samples, we found DNA evidence of a novel borrelia of uncertain significance in one, which was also positive for the 2-tier serology test. The rest of the post-treatment sera and all 20 control sera were PCR-negative. Of the 20 pre-treatment sera from clinically suspect early Lyme disease patients, we found Borrelia miyamotoi in one which was 2-tier serology-negative, and a Borrelia burgdorferi in two-one negative and one positive for 2-tier serology. We conclude that a sensitive and reliable DNA-based test is needed to support the diagnosis of Lyme disease and Lyme disease-like borreliosis.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.