ArticlePloS one2014
Comparative proteomic analysis of experimental evolution of the Bacillus cereus-Ketogulonicigenium vulgare co-culture.
Article in PloS one, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
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Who cites it
11 citing papers in PubMed, 20 citations in OpenAlex.
- Deciphering the Link Between Gut Bacterial Community Structure of Forest Musk Deer (Veterinary sciences · 2026Article
- The industrial versatility of Gluconobacter oxydans: current applications and future perspectives.World journal of microbiology & biotechnology · 2022Review
- Construction of synthetic microbial consortia for 2-keto-L-gulonic acid biosynthesis.Synthetic and systems biotechnology · 2022Article
- Integrated proteomic and metabolomic analysis of a reconstructed three-species microbial consortium for one-step fermentation of 2-keto-L-gulonic acid, the precursor of vitamin C.Journal of industrial microbiology & biotechnology · 2019Article
- Fermentation Titer Optimization and Impact on Energy and Water Consumption during Downstream Processing.Chemical engineering & technology · 2018Article
- Comparative genomics and metabolomics analyses of the adaptation mechanism in Ketogulonicigenium vulgare-Bacillus thuringiensis consortium.Scientific reports · 2017Article
- Insights into mutualism mechanism and versatile metabolism of Ketogulonicigenium vulgare Hbe602 based on comparative genomics and metabolomics studies.Scientific reports · 2016Article
- Unraveling interactions in microbial communities - from co-cultures to microbiomes.Journal of microbiology (Seoul, Korea) · 2015Review
- Elucidating Host-Pathogen Interactions Based on Post-Translational Modifications Using Proteomics Approaches.Frontiers in microbiology · 2015Review
- Article
- Metabolomic analysis of cooperative adaptation between co-cultured Bacillus cereus and Ketogulonicigenium vulgare.PloS one · 2014Article
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The microbial co-culture system composing of Ketogulonicigenium vulgare and Bacillus cereus was widely adopted in industry for the production of 2-keto-gulonic acid (2-KGA), the precursor of vitamin C. We found serial subcultivation of the co-culture could enhance the yield of 2-KGA by 16% in comparison to that of the ancestral co-culture. To elucidate the evolutionary dynamics and interaction mechanisms of the two microbes, we performed iTRAQ-based quantitative proteomic analyses of the pure cultures of K. vulgare, B. cereus and their co-culture during serial subcultivation. Hierarchy cluster analyses of the proteomic data showed that the expression level of a number of crucial proteins associated with sorbose conversion and oligopeptide transport was significantly enhanced by the experimental evolution. In particular, the expression level of sorbose/sorbosone dehydrogenase was enhanced in the evolved K. vulgare, while the expression level of InhA and the transport efficiency of oligopeptides were increased in the evolved B. cereus. The decreased sporulating protein expression and increased peptide transporter expression observed in evolved B. cereus, together with the increased amino acids synthesis in evolved K. vulgare suggested that serial subcultivation result in enhanced synergistic cooperation between K. vulgare and B. cereus, enabling an increased production of 2-KGA.
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