ArticleScientific reports2014
Ratiometric electrochemical proximity assay for sensitive one-step protein detection.
Article in Scientific reports, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed.
- Ultrasensitive non-enzymatic protein detection using proximity immunoassay with photonic resonator absorption microscopy.Npj biosensing · 2026Article
- Extended Review Concerning the Integration of Electrochemical Biosensors into Modern IoT and Wearable Devices.Biosensors · 2024Review
- Rationally Designed DNA-Based Scaffolds and Switching Probes for Protein Sensing.Advances in biochemical engineering/biotechnology · 2024Review
- Synthesis of Site-Specific Antibody-[60]Fullerene-Oligonucleotide Conjugates for Cellular Targeting.ACS applied bio materials · 2023Article
- Device integration of electrochemical biosensors.Nature reviews bioengineering · 2023Review
- Ratiometric Electrochemistry: Improving the Robustness, Reproducibility and Reliability of Biosensors.Molecules (Basel, Switzerland) · 2021Review
- Nucleic-Acid Driven Cooperative Bioassays Using Probe Proximity or Split-Probe Techniques.Analytical chemistry · 2021Review
- An electrochemical aptasensor for amyloid-β oligomer based on double-stranded DNA as "conductive spring".Mikrochimica acta · 2020Article
- Nonfaradaic Current Suppression in DNA-Based Electrochemical Assays with a Differential Potentiostat.Analytical chemistry · 2019Article
- Nucleic acid-based ratiometric electrochemiluminescent, electrochemical and photoelectrochemical biosensors: a review.Mikrochimica acta · 2019Review
- Development of the covalent antibody-DNA conjugates technology for detection of IgE and IgM antibodies by immuno-PCR.PloS one · 2019Article
- An electrochemical method for sensitive and rapid detection of FAM134B protein in colon cancer samples.Scientific reports · 2017Article
- Ratiometric ultrasensitive electrochemical immunosensor based on redox substrate and immunoprobe.Scientific reports · 2016Article
- Advances in biosensing strategies for HIV-1 detection, diagnosis, and therapeutic monitoring.Advanced drug delivery reviews · 2016Review
- A wavelength-resolved ratiometric photoelectrochemical technique: design and sensing applications.Chemical science · 2016Article
- Smart protein biogate as a mediator to regulate competitive host-guest interaction for sensitive ratiometric electrochemical assay of prion.Scientific reports · 2015Article
- Quantifying Aptamer-Protein Binding via Thermofluorimetric Analysis.Analytical methods : advancing methods and applications · 2015Article
- High-throughput imaging assay of multiple proteinsChemical science · 2015Article
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Authors and funding
4 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
This work proposes the concept of ratiometric electrochemical proximity assay (REPA), which can be used for one-step, highly sensitive and selective detection of protein. The assay strategy was achieved on a sensing interface that was formed by hybridization of methylene blue (MB)-labeled antibody-DNA probe (MB-DNA1-Ab1) with ferrocene (Fc)-labeled DNA capture probe (Fc-P) modified gold electrode. On the interface the target protein could trigger the formation of immunocomplex between MB-DNA1-Ab1 and detection antibody-DNA probe (Ab2-DNA2) and subsequently the proximity hybridization of DNA1-DNA2, which led to the departure of MB-DNA1-Ab1 from the interface. The remained Fc-P could form a hairpin structure to take Fc group to electrode surface. Therefore, the recognition of target protein to Ab1 and Ab2 resulted in both the "signal-off" of MB and the "signal-on" of Fc for dual-signal electrochemical ratiometric readout. The proposed REPA could be carried out in one-step with 40-min duration and showed a wide detection range from 0.05 to 100 ng/mL with pg/mL limit of detection, displaying great potential for convenient point-of-care testing and commercial application.
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