ArticleThe FEBS journal2014
The substrate specificity, enantioselectivity and structure of the (R)-selective amine : pyruvate transaminase from Nectria haematococca.
Article in The FEBS journal, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 66 citations in OpenAlex.
- Functional and structural insights into a thermostable (S)-selective amine transaminase and its improved substrate scope by protein engineering.Applied microbiology and biotechnology · 2025Article
- Protein engineering of transaminase facilitating enzyme cascade reaction for the biosynthesis of azasugars.iScience · 2024Article
- To the Understanding of Catalysis by D-Amino Acid Transaminases: A Case Study of the Enzyme fromMolecules (Basel, Switzerland) · 2023Article
- Non-Canonical Amino Acid-Based Engineering of (Frontiers in chemistry · 2022Article
- Identification, Characterization, and Site-Specific Mutagenesis of a Thermostable ω-Transaminase fromACS omega · 2021Article
- Expanding the Toolbox of R-Selective Amine Transaminases by Identification and Characterization of New Members.Chembiochem : a European journal of chemical biology · 2021Article
- Synthesis of Sitagliptin Intermediate by a Multi-Enzymatic Cascade System Using Lipase and Transaminase With Benzylamine as an Amino Donor.Frontiers in bioengineering and biotechnology · 2021Article
- Transaminases for industrial biocatalysis: novel enzyme discovery.Applied microbiology and biotechnology · 2020Review
- An (Frontiers in bioengineering and biotechnology · 2020Article
- A Single Mutation Increases the Thermostability and Activity ofMolecules (Basel, Switzerland) · 2019Article
- Bioprospecting Reveals Class III ω-Transaminases Converting Bulky Ketones and Environmentally Relevant Polyamines.Applied and environmental microbiology · 2019Article
- Thermostable Branched-Chain Amino Acid Transaminases From the ArchaeaFrontiers in bioengineering and biotechnology · 2019Article
- Discovery and structural characterisation of new fold type IV-transaminases exemplify the diversity of this enzyme fold.Scientific reports · 2016Article
- Identification of (S)-selective transaminases for the asymmetric synthesis of bulky chiral amines.Nature chemistry · 2016Article
- The Industrial Age of Biocatalytic Transamination.European journal of organic chemistry · 2015Article
- A new target region for changing the substrate specificity of amine transaminases.Scientific reports · 2015Article
Corrections and comments
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Authors and funding
7 authors at 2 institutions in 1 country.
Funding
Abstract
unlabelledDuring the last decade the use of transaminases for the production of pharmaceutical and fine chemical intermediates has attracted a great deal of attention. Transaminases are versatile biocatalysts for the efficient production of amine intermediates and many have (S)-enantiospecificity. Transaminases with (R)-specificity are needed to expand the applications of these enzymes in biocatalysis. In this work we have identified a fungal putative (R)-specific transaminase from the Eurotiomycetes Nectria haematococca, cloned a synthetic version of this gene, demonstrated (R)-selective deamination of several substrates including (R)-α-methylbenzylamine, as well as production of (R)-amines, and determined its crystal structure. The crystal structures of the holoenzyme and the complex with an inhibitor gabaculine offer the first detailed insight into the structural basis for substrate specificity and enantioselectivity of the industrially important class of (R)-selective amine : pyruvate transaminases. DATABASE: The atomic coordinates and structure factors for the Nectria TAm in holoenzyme and gabaculine-bound forms have been deposited in the PDB as entries 4cmd and 4cmf respectively.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.