Evidence map›Paper›PMID 24616553›Full record

ArticleMediators of inflammation2014

Differences in the antigens of Helicobacter pylori strains influence on the innate immune response in the in vitro experiments.

Miha Skvarc, Andreja Natasa Kopitar, Janko Kos, Natasa Obermajer, Bojan Tepes

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Mediators of inflammation, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
0.3field-weighted citation impact, top 38% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed, 4 citations in OpenAlex.

  1. Review
  2. The Relationship between Toll-like Receptors andJournal of immunology research · 2019
    Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors at 2 institutions in 1 country.

Miha SkvarcInstitute of Microbiology and Immunology, Medical Faculty Ljubljana, University of Ljubljana, Zaloska 4, 1000 Ljubljana, Slovenia.ORCID 0000-0003-0170-1733
Andreja Natasa KopitarInstitute of Microbiology and Immunology, Medical Faculty Ljubljana, University of Ljubljana, Zaloska 4, 1000 Ljubljana, Slovenia.
Janko KosDepartment of Pharmaceutical Biology, Faculty of Pharmacy, University of Ljubljana, Askerceva 7, 1000 Ljubljana, Slovenia ; Department of Biotechnology, Jozef Stefan Institute, Jamova 39, 1000 Ljubljana, Slovenia.
Natasa ObermajerDepartment of Pharmaceutical Biology, Faculty of Pharmacy, University of Ljubljana, Askerceva 7, 1000 Ljubljana, Slovenia.
Bojan TepesAM-Diagnostic Centre Rogaska, Prvomajska ulica 29A, 3250 Rogaska Slatina, Slovenia.
University of Ljubljana · SIRogaška Medical Centre · SI

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

The immune response to Helicobacter pylori importantly determines the pathogenesis of infection as well as the success of antibiotic eradication of the bacteria. Strains of H. pylori were gathered from 14 patients who failed to eradicate H. pylori infection with antibiotics-therapy resistant strains (TRS)-or from patients who were able to eradicate H. pylori infection-therapy susceptible strains (TSS). The THP-1 cells were stimulated with H. pylori antigens. Cathepsin X expression on THP-1 cells and concentration of cytokines in the supernatant of THP-1 cells were measured with a flow cytometer. TSS H. pylori antigens increased the proportion of cathepsin X positive cells compared to TRS H. pylori antigens. TSS H. pylori antigens induced higher secretion of IL-12 and IL-6 compared to TRS H. pylori antigens (P < 0.001; 0.02). Polymyxin B, a lipid A inhibitor, lowered the secretion of IL-12 and IL-6 in TRS and TSS. We demonstrated a H. pylori strain-dependent cathepsin X and cytokine expression that can be associated with H. pylori resistance to eradication due to lack of effective immune response. Differences in lipid A of H. pylori might have an influence on the insufficient immune response, especially on phagocytosis.

Indexed as

Antigens, BacterialCathepsinsCell LineCytokinesDyspepsiaFlow CytometryGastric MucosaHelicobacter pyloriHumansImmunity, InnateInterleukin-12Interleukin-6Lipid ALipopolysaccharidesMacrophagesPolymyxin BAntigens, BacterialCathepsinsCytokinesInterleukin-12Interleukin-6Lipid ALipopolysaccharidesPolymyxin Bprocathepsin X, humanProtein Precursors

Identifiers

PMID24616553
PMCPMC3927579
OpenAlexW2019189578

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.